In vivo micronucleus assay and GST activity in assessing genotoxicity of plumbagin in Swiss albino mice

V. SivaKumar, Prakash Radhakrishnan, M. R. Murali, H. Devaraj, S. Niranjali Devaraj

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Information available on the mutagenicity of a large number of indigenous drugs commonly employed in the Siddha and Ayurveda systems of medicine is scanty. In this context, the current investigation on plumbagin, 5-hydroxy-2methyl-1,4-napthoquinone, an active principle in the roots of Plumbago zeylanica used in Siddha and Ayurveda for various ailments, was carried out; 16 mg/kg b.w. (LD50) was fixed as the maximum dose. Subsequent dose levels were fixed as 50% and 25% of LD50 amounting to 8 mg and 4 mg/kg b.w., respectively, and given orally for 5 consecutive days in 1% Carboxyl methyl cellulose (CMC) to Swiss albino mice weighing 25-30 g. The micronucleus assay was done in mouse bone marrow. Plumbagin was found to induce micronuclei at all the doses studied (4 mg/kg, 8 mg/kg, 16 mg/kg b.w.), and it proves to be toxic to bone marrow cells of Swiss albino mice. Animal treated with cyclophosphamide (40 mg/kg b.w.) served as positive control. In addition, glutathione S-transferase (GST) activity was observed in control, plumbagin (4 mg, 8 mg, 16 mg/kg b.w., respectively), and genotoxin-treated experimental group of animals. No significant change in GST activity was observed with plumbagin dose of 4 mg/kg b.w., whereas GST activity was significantly inhibited by higher doses of plumbagin (8 mg and 16 mg/ kg b.w.) and cyclophosphamide.

Original languageEnglish (US)
Pages (from-to)499-507
Number of pages9
JournalDrug and Chemical Toxicology
Volume28
Issue number4
DOIs
StatePublished - Nov 15 2005
Externally publishedYes

Fingerprint

Micronucleus Tests
Glutathione Transferase
Assays
Bone
Animals
Lethal Dose 50
Cyclophosphamide
Weighing
Plumbaginaceae
Medicine
Cellulose
Cells
Methylcellulose
Poisons
Mutagens
Systems Analysis
Bone Marrow Cells
Bone Marrow
plumbagin
Glutathione

Keywords

  • Cyclophosphamide
  • Genotoxicity
  • Micronucleus test
  • Plumbagin

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Cite this

In vivo micronucleus assay and GST activity in assessing genotoxicity of plumbagin in Swiss albino mice. / SivaKumar, V.; Radhakrishnan, Prakash; Murali, M. R.; Devaraj, H.; Devaraj, S. Niranjali.

In: Drug and Chemical Toxicology, Vol. 28, No. 4, 15.11.2005, p. 499-507.

Research output: Contribution to journalArticle

SivaKumar, V. ; Radhakrishnan, Prakash ; Murali, M. R. ; Devaraj, H. ; Devaraj, S. Niranjali. / In vivo micronucleus assay and GST activity in assessing genotoxicity of plumbagin in Swiss albino mice. In: Drug and Chemical Toxicology. 2005 ; Vol. 28, No. 4. pp. 499-507.
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