In vivo and in vitro anaerobic mating in Candida albicans

Raluca Dumitru, Dhammika H.M.L.P. Navarathna, Camile P. Semighini, Christian G. Elowsky, Razvan V. Dumitru, Daniel Dignard, Malcolm Whiteway, Audrey L. Atkin, Kenneth W. Nickerson

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Candida albicans cells of opposite mating types are thought to conjugate during infection in mammalian hosts, but paradoxically, the mating-competent opaque state is not stable at mammalian body temperatures. We found that anaerobic conditions stabilize the opaque state at 37°C, block production of famesol, and permit in vitro mating at 37°C at efficiencies of up to 84%. Aerobically, farnesol prevents mating because it kills the opaque cells necessary for mating, and as a corollary, farnesol production is turned off in opaque cells. These in vitro observations suggest that naturally anaerobic sites, such as the efficiently colonized gastrointestinal (GI) tract, could serve as niches for C. albicans mating. In a direct test of mating in the mouse GI tract, prototrophic cells were obtained from auxotrophic parent cells, confirming that mating will occur in this organ. These cells were true mating products because they were tetraploid, mononuclear, and prototrophic, and they contained the heterologous hisG marker from one of the parental strains.

Original languageEnglish (US)
Pages (from-to)465-472
Number of pages8
JournalEukaryotic Cell
Volume6
Issue number3
DOIs
StatePublished - Mar 1 2007

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Candida albicans
Farnesol
farnesol
cells
gastrointestinal system
Gastrointestinal Tract
Tetraploidy
Body Temperature
anaerobic conditions
body temperature
tetraploidy
In Vitro Techniques
niches
mice
Infection
infection
testing

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Dumitru, R., Navarathna, D. H. M. L. P., Semighini, C. P., Elowsky, C. G., Dumitru, R. V., Dignard, D., ... Nickerson, K. W. (2007). In vivo and in vitro anaerobic mating in Candida albicans. Eukaryotic Cell, 6(3), 465-472. https://doi.org/10.1128/EC.00316-06

In vivo and in vitro anaerobic mating in Candida albicans. / Dumitru, Raluca; Navarathna, Dhammika H.M.L.P.; Semighini, Camile P.; Elowsky, Christian G.; Dumitru, Razvan V.; Dignard, Daniel; Whiteway, Malcolm; Atkin, Audrey L.; Nickerson, Kenneth W.

In: Eukaryotic Cell, Vol. 6, No. 3, 01.03.2007, p. 465-472.

Research output: Contribution to journalArticle

Dumitru, R, Navarathna, DHMLP, Semighini, CP, Elowsky, CG, Dumitru, RV, Dignard, D, Whiteway, M, Atkin, AL & Nickerson, KW 2007, 'In vivo and in vitro anaerobic mating in Candida albicans', Eukaryotic Cell, vol. 6, no. 3, pp. 465-472. https://doi.org/10.1128/EC.00316-06
Dumitru R, Navarathna DHMLP, Semighini CP, Elowsky CG, Dumitru RV, Dignard D et al. In vivo and in vitro anaerobic mating in Candida albicans. Eukaryotic Cell. 2007 Mar 1;6(3):465-472. https://doi.org/10.1128/EC.00316-06
Dumitru, Raluca ; Navarathna, Dhammika H.M.L.P. ; Semighini, Camile P. ; Elowsky, Christian G. ; Dumitru, Razvan V. ; Dignard, Daniel ; Whiteway, Malcolm ; Atkin, Audrey L. ; Nickerson, Kenneth W. / In vivo and in vitro anaerobic mating in Candida albicans. In: Eukaryotic Cell. 2007 ; Vol. 6, No. 3. pp. 465-472.
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