In vitro transcription of ribosomal RNA on phage λrifd 18 DNA

Ibolya Kiss, Krystina Slaska, János Sümegi, Andor Udvardy, Pál Venetianer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In vitro transcription of ribosomal RNA was studied on the DNA of the transducing bacteriophage λrifd 18, which carries an rRNA transcription unit from Escherichia coli. rRNA synthesis was preferential at all polymerase DNA ratios tested, and at the optimal 0.3 weight ratio nearly 60% of the transcript was rRNA. At this ratio the principal product of transcription comigrated in acrylamide-agarose electrophoresis with authentic 30-S rRNA precursor synthesized in vivo. In the presence of rifampicin more than one equivalent of rRNA was synthesized, thus suggesting the existence of two initiation sites for rRNA on the phage DNA. Similar results were obtained on E. coli DNA. Preincubation with heparin virtually eliminated the transcription of rRNA, in sharp contrast with the results of similar experiments on bacterial DNA where rRNA genes were transcribed 4-5 times in the presence of heparin. The possible explanation of this difference between rRNA promotors on the phage and the bacterial DNA are discussed.

Original languageEnglish (US)
Pages (from-to)257-266
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume518
Issue number2
DOIs
StatePublished - Apr 27 1978

Fingerprint

RNA Phages
Ribosomal RNA
Bacteriophages
Bacterial DNA
Heparin
DNA
Escherichia coli
Acrylamide
RNA Precursors
DNA-Directed DNA Polymerase
Rifampin
rRNA Genes
Sepharose
Electrophoresis
Weights and Measures
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

In vitro transcription of ribosomal RNA on phage λrifd 18 DNA. / Kiss, Ibolya; Slaska, Krystina; Sümegi, János; Udvardy, Andor; Venetianer, Pál.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 518, No. 2, 27.04.1978, p. 257-266.

Research output: Contribution to journalArticle

Kiss, Ibolya ; Slaska, Krystina ; Sümegi, János ; Udvardy, Andor ; Venetianer, Pál. / In vitro transcription of ribosomal RNA on phage λrifd 18 DNA. In: BBA Section Nucleic Acids And Protein Synthesis. 1978 ; Vol. 518, No. 2. pp. 257-266.
@article{366cfef78c754e55be61538c14d73ad0,
title = "In vitro transcription of ribosomal RNA on phage λrifd 18 DNA",
abstract = "In vitro transcription of ribosomal RNA was studied on the DNA of the transducing bacteriophage λrifd 18, which carries an rRNA transcription unit from Escherichia coli. rRNA synthesis was preferential at all polymerase DNA ratios tested, and at the optimal 0.3 weight ratio nearly 60{\%} of the transcript was rRNA. At this ratio the principal product of transcription comigrated in acrylamide-agarose electrophoresis with authentic 30-S rRNA precursor synthesized in vivo. In the presence of rifampicin more than one equivalent of rRNA was synthesized, thus suggesting the existence of two initiation sites for rRNA on the phage DNA. Similar results were obtained on E. coli DNA. Preincubation with heparin virtually eliminated the transcription of rRNA, in sharp contrast with the results of similar experiments on bacterial DNA where rRNA genes were transcribed 4-5 times in the presence of heparin. The possible explanation of this difference between rRNA promotors on the phage and the bacterial DNA are discussed.",
author = "Ibolya Kiss and Krystina Slaska and J{\'a}nos S{\"u}megi and Andor Udvardy and P{\'a}l Venetianer",
year = "1978",
month = "4",
day = "27",
doi = "10.1016/0005-2787(78)90182-X",
language = "English (US)",
volume = "518",
pages = "257--266",
journal = "BBA Section Nucleic Acids And Protein Synthesis",
issn = "0005-2787",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - In vitro transcription of ribosomal RNA on phage λrifd 18 DNA

AU - Kiss, Ibolya

AU - Slaska, Krystina

AU - Sümegi, János

AU - Udvardy, Andor

AU - Venetianer, Pál

PY - 1978/4/27

Y1 - 1978/4/27

N2 - In vitro transcription of ribosomal RNA was studied on the DNA of the transducing bacteriophage λrifd 18, which carries an rRNA transcription unit from Escherichia coli. rRNA synthesis was preferential at all polymerase DNA ratios tested, and at the optimal 0.3 weight ratio nearly 60% of the transcript was rRNA. At this ratio the principal product of transcription comigrated in acrylamide-agarose electrophoresis with authentic 30-S rRNA precursor synthesized in vivo. In the presence of rifampicin more than one equivalent of rRNA was synthesized, thus suggesting the existence of two initiation sites for rRNA on the phage DNA. Similar results were obtained on E. coli DNA. Preincubation with heparin virtually eliminated the transcription of rRNA, in sharp contrast with the results of similar experiments on bacterial DNA where rRNA genes were transcribed 4-5 times in the presence of heparin. The possible explanation of this difference between rRNA promotors on the phage and the bacterial DNA are discussed.

AB - In vitro transcription of ribosomal RNA was studied on the DNA of the transducing bacteriophage λrifd 18, which carries an rRNA transcription unit from Escherichia coli. rRNA synthesis was preferential at all polymerase DNA ratios tested, and at the optimal 0.3 weight ratio nearly 60% of the transcript was rRNA. At this ratio the principal product of transcription comigrated in acrylamide-agarose electrophoresis with authentic 30-S rRNA precursor synthesized in vivo. In the presence of rifampicin more than one equivalent of rRNA was synthesized, thus suggesting the existence of two initiation sites for rRNA on the phage DNA. Similar results were obtained on E. coli DNA. Preincubation with heparin virtually eliminated the transcription of rRNA, in sharp contrast with the results of similar experiments on bacterial DNA where rRNA genes were transcribed 4-5 times in the presence of heparin. The possible explanation of this difference between rRNA promotors on the phage and the bacterial DNA are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0017904063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017904063&partnerID=8YFLogxK

U2 - 10.1016/0005-2787(78)90182-X

DO - 10.1016/0005-2787(78)90182-X

M3 - Article

C2 - 350278

AN - SCOPUS:0017904063

VL - 518

SP - 257

EP - 266

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

SN - 0005-2787

IS - 2

ER -