In vitro tramsformation of rat bladder epithelium by 2-amino-4-(5-nitro-2-furyl)thiazole

Angela M. Mann, Tsuneo Masui, Frank J. Chlapowski, Takehiko Okamura, Claudia D. Borgeson, Samuel Monroe Cohen

Research output: Contribution to journalArticle

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Abstract

To establish a rat urinary bladder carcinogenesis model in vitro, primary rat bladder epithelial cells were grown in media containing 2-amino-4(5-nitro-2-furyl)thiazole (ANFT), the water-soluble metabolite of N-[4-(5-nitro-2-furyl)-2-thiazolyl]-formamide (FANFT), for 4 weeks followed by longterm (4-7 months) exposure to control medium, sodium saccharin (NaS), or urea. Another set of cultures were exposed to ANFT, NaS and urea simultaneously. Several phenotypic changes were observed in the chemically exposed cell cultures, namely differences in cell morphology, increased growth rate and the ability to grow on plastic instead of rattail collagen support. All of the chemically exposed cultures were anchorage independent except one of those treated with NaS. The ANFT-treated cells followed by control medium or urea and cells treated with ANFT, NaS and urea were tumorigenic when transplanted to nude mice, whereas NaS or ANFT followed by NaS treatment were not. The tumors were carcinomas and their epithelial differentiation was verified by strong positive staining for cytokeratin. These studies demonstrate the urothelial transforming capability of ANFT in cell culture without the necessity for a long exposure to a secondary chemical.

Original languageEnglish (US)
Pages (from-to)417-422
Number of pages6
JournalCarcinogenesis
Volume12
Issue number3
DOIs
StatePublished - Mar 1 1991

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Urinary Bladder
Epithelium
Urea
FANFT
Cell Culture Techniques
Saccharin
Keratins
In Vitro Techniques
ANFT
Nude Mice
Plastics
Carcinogenesis
Collagen
Epithelial Cells
Staining and Labeling
Carcinoma
Water
Growth
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

Cite this

In vitro tramsformation of rat bladder epithelium by 2-amino-4-(5-nitro-2-furyl)thiazole. / Mann, Angela M.; Masui, Tsuneo; Chlapowski, Frank J.; Okamura, Takehiko; Borgeson, Claudia D.; Cohen, Samuel Monroe.

In: Carcinogenesis, Vol. 12, No. 3, 01.03.1991, p. 417-422.

Research output: Contribution to journalArticle

Mann, AM, Masui, T, Chlapowski, FJ, Okamura, T, Borgeson, CD & Cohen, SM 1991, 'In vitro tramsformation of rat bladder epithelium by 2-amino-4-(5-nitro-2-furyl)thiazole', Carcinogenesis, vol. 12, no. 3, pp. 417-422. https://doi.org/10.1093/carcin/12.3.417
Mann, Angela M. ; Masui, Tsuneo ; Chlapowski, Frank J. ; Okamura, Takehiko ; Borgeson, Claudia D. ; Cohen, Samuel Monroe. / In vitro tramsformation of rat bladder epithelium by 2-amino-4-(5-nitro-2-furyl)thiazole. In: Carcinogenesis. 1991 ; Vol. 12, No. 3. pp. 417-422.
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