In vitro synthesis of heat-shock proteins by mRNAs from chicken embryo fibroblasts.

Philip M Kelley, G. Aliperti, M. J. Schlesinger

Research output: Contribution to journalArticle

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Abstract

The pattern of proteins synthesized by chicken embryo fibroblasts changes dramatically after these cells are incubated at 45 degrees C for a few hours. Three proteins (Mr = 22,000, 76,000, and 95,000) account for almost 50% of the cell's protein synthetic capacity immediately after the heat-shock (Kelley, P.M., and Schlesinger, M.J. (1978) Cell 15, 1277-1286). When mRNAs were isolated from heat-shocked cells and translated in a cell-free protein synthesizing system, a pattern of proteins virtually identical with that made by intact heat-shocked cells was detected. Mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and radioimmune precipitation with specific antisera were used to establish the identity of in vitro- and in vivo-generated heat-shock proteins. The mRNAs coding for the major heat-shock proteins could be separated by rate zonal centrifugation in a sucrose gradient and mRNAs with sedimentation coefficients of 20 S, 18 S, and 13 S were translated in vitro to yield proteins of 95, 76, and 22 kilodaltons, respectively.

Original languageEnglish (US)
Pages (from-to)3230-3233
Number of pages4
JournalJournal of Biological Chemistry
Volume255
Issue number8
StatePublished - Apr 25 1980

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Fibroblasts
Heat-Shock Proteins
Chickens
Embryonic Structures
Messenger RNA
Proteins
Hot Temperature
Zonal Centrifugation
Artificial Cells
Centrifugation
Electrophoresis
Sedimentation
Sodium Dodecyl Sulfate
Sucrose
In Vitro Techniques
Immune Sera
Polyacrylamide Gel Electrophoresis
Shock

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Kelley, P. M., Aliperti, G., & Schlesinger, M. J. (1980). In vitro synthesis of heat-shock proteins by mRNAs from chicken embryo fibroblasts. Journal of Biological Chemistry, 255(8), 3230-3233.

In vitro synthesis of heat-shock proteins by mRNAs from chicken embryo fibroblasts. / Kelley, Philip M; Aliperti, G.; Schlesinger, M. J.

In: Journal of Biological Chemistry, Vol. 255, No. 8, 25.04.1980, p. 3230-3233.

Research output: Contribution to journalArticle

Kelley, PM, Aliperti, G & Schlesinger, MJ 1980, 'In vitro synthesis of heat-shock proteins by mRNAs from chicken embryo fibroblasts.', Journal of Biological Chemistry, vol. 255, no. 8, pp. 3230-3233.
Kelley, Philip M ; Aliperti, G. ; Schlesinger, M. J. / In vitro synthesis of heat-shock proteins by mRNAs from chicken embryo fibroblasts. In: Journal of Biological Chemistry. 1980 ; Vol. 255, No. 8. pp. 3230-3233.
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