In vitro propagation of a Himalayan pine P. wallichiana A. B. Jacks

Dhundy Raj Bastola, V. P. Agrawal, Nirmal Joshee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Four different plant tissue culture media containing varying levels of growth hormones were studied to determine the nutritional and hormonal requirements for induction and subsequent elongation of shoot buds and rooting. Germinating embryos as explants placed in an inverted position in LP medium containing BAP produced adventitious shoot buds. Activated charcoal treatment of the embryos after being exposed to BAP was necessary for shoot proliferation and elongation. Elongated shoot buds were passed to rooting media. While using this technique for micropropagation of other pines, it would be necessary to optimize the age of the explant and the duration of exposure in shoot induction medium.

Original languageEnglish (US)
Pages (from-to)338-341
Number of pages4
JournalCurrent Science
Volume78
Issue number3
StatePublished - Feb 10 2000

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Embryonic Structures
Pinus
Nutritional Requirements
shoots
buds
Charcoal
embryo (plant)
Growth Hormone
Culture Media
rooting
explants
activated carbon
adventitious shoots
micropropagation
somatotropin
exposure duration
tissue culture
plant tissues
culture media
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

In vitro propagation of a Himalayan pine P. wallichiana A. B. Jacks. / Bastola, Dhundy Raj; Agrawal, V. P.; Joshee, Nirmal.

In: Current Science, Vol. 78, No. 3, 10.02.2000, p. 338-341.

Research output: Contribution to journalArticle

Bastola, DR, Agrawal, VP & Joshee, N 2000, 'In vitro propagation of a Himalayan pine P. wallichiana A. B. Jacks', Current Science, vol. 78, no. 3, pp. 338-341.
Bastola, Dhundy Raj ; Agrawal, V. P. ; Joshee, Nirmal. / In vitro propagation of a Himalayan pine P. wallichiana A. B. Jacks. In: Current Science. 2000 ; Vol. 78, No. 3. pp. 338-341.
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