In vitro myelin formation using embryonic stem cells

Bilal E. Kerman, Hyung Joon Kim, Krishnan Padmanabhan, Arianna Mei, Shereen Georges, Matthew S. Joens, James A.J. Fitzpatrick, Roberto Jappelli, Karen J. Chandross, Paul August, Fred H. Gage

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Myelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation.

Original languageEnglish (US)
Pages (from-to)2213-2225
Number of pages13
JournalDevelopment (Cambridge)
Volume142
Issue number12
DOIs
StatePublished - Jun 15 2015

Fingerprint

Embryonic Stem Cells
Myelin Sheath
Oligodendroglia
Lab-On-A-Chip Devices
Neurons
Axons
Central Nervous System
In Vitro Techniques
Brain

Keywords

  • Automated quantification
  • Embryonic stem cells
  • Live imaging
  • Myelin
  • Oligodendrocyte

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Kerman, B. E., Kim, H. J., Padmanabhan, K., Mei, A., Georges, S., Joens, M. S., ... Gage, F. H. (2015). In vitro myelin formation using embryonic stem cells. Development (Cambridge), 142(12), 2213-2225. https://doi.org/10.1242/dev.116517

In vitro myelin formation using embryonic stem cells. / Kerman, Bilal E.; Kim, Hyung Joon; Padmanabhan, Krishnan; Mei, Arianna; Georges, Shereen; Joens, Matthew S.; Fitzpatrick, James A.J.; Jappelli, Roberto; Chandross, Karen J.; August, Paul; Gage, Fred H.

In: Development (Cambridge), Vol. 142, No. 12, 15.06.2015, p. 2213-2225.

Research output: Contribution to journalArticle

Kerman, BE, Kim, HJ, Padmanabhan, K, Mei, A, Georges, S, Joens, MS, Fitzpatrick, JAJ, Jappelli, R, Chandross, KJ, August, P & Gage, FH 2015, 'In vitro myelin formation using embryonic stem cells', Development (Cambridge), vol. 142, no. 12, pp. 2213-2225. https://doi.org/10.1242/dev.116517
Kerman BE, Kim HJ, Padmanabhan K, Mei A, Georges S, Joens MS et al. In vitro myelin formation using embryonic stem cells. Development (Cambridge). 2015 Jun 15;142(12):2213-2225. https://doi.org/10.1242/dev.116517
Kerman, Bilal E. ; Kim, Hyung Joon ; Padmanabhan, Krishnan ; Mei, Arianna ; Georges, Shereen ; Joens, Matthew S. ; Fitzpatrick, James A.J. ; Jappelli, Roberto ; Chandross, Karen J. ; August, Paul ; Gage, Fred H. / In vitro myelin formation using embryonic stem cells. In: Development (Cambridge). 2015 ; Vol. 142, No. 12. pp. 2213-2225.
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