In vitro inhibition of trophoblast maturation and expansion of early rat blastocysts by an oestrogen antagonist

S. K. Roy, J. Sengupta, B. C. Paria, S. K. Manchanda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Rat blastocyst development and expansion were studied in an in vitro culture system. Early blastocysts collected from rats on the morning of day 5 were shown to develop normally and underwent expansion when placed in bovine serum ablumin supplemented culture medium for 12 h at 37°C. Nafoxidine, and oestrogen antagonist, prevented embryo expansion when added to the culture medium, and it was found that if oestradiol-17β was added to this medium this inhibitory effect was removed and the embryos again expanded. Ethanol, used as a solvent for steroids, and cholesterol were added to the medium containing nafoxidine but the blastocysts still remained unexpanded after 12 h in culture. These results suggest a need for oestrogen action during blastocyst expansion in vitro. Cultured rat blastocysts were subjected to histochemical study for acid phosphatase (ACPase) and leucylnaphthylamidase (LNAse). In unexpanded blastocysts ACPase was found to be low while LNAse activity was higher when compared with enzyme distribution patterns in expanded blastocysts.

Original languageEnglish (US)
Pages (from-to)129-135
Number of pages7
JournalActa Endocrinologica
Volume99
Issue number1
StatePublished - Jan 1 1982

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Estrogen Antagonists
Trophoblasts
Blastocyst
Nafoxidine
Acid Phosphatase
Culture Media
Embryonic Structures
In Vitro Techniques
Estradiol
Estrogens
Ethanol
Steroids
Cholesterol
Enzymes
Serum

ASJC Scopus subject areas

  • Endocrinology

Cite this

In vitro inhibition of trophoblast maturation and expansion of early rat blastocysts by an oestrogen antagonist. / Roy, S. K.; Sengupta, J.; Paria, B. C.; Manchanda, S. K.

In: Acta Endocrinologica, Vol. 99, No. 1, 01.01.1982, p. 129-135.

Research output: Contribution to journalArticle

Roy, S. K. ; Sengupta, J. ; Paria, B. C. ; Manchanda, S. K. / In vitro inhibition of trophoblast maturation and expansion of early rat blastocysts by an oestrogen antagonist. In: Acta Endocrinologica. 1982 ; Vol. 99, No. 1. pp. 129-135.
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