In vitro digestion and characterisation of 2S albumin and digestion-resistant peptides in pecan

Jelena Spiric, Stef J. Koppelman, Andre Knulst, Julie A. Nordlee, Steve L. Taylor, Joseph L. Baumert

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The 2S albumins are one of the major protein families involved in severe food allergic reactions to nuts, seeds and legumes, thus potentially making these proteins clinically relevant for allergic sensitisation and potential diagnostic markers. In this study, we sought to purify native 2S albumin protein from pecan to further characterise this putative allergen. The purified 2S albumin, Car i 1, from pecan was found to be resistant to digestion by pepsin in simulated gastric fluid (SGF) and comparatively stable to proteolysis by trypsin and pancreatin in simulated intestinal fluid (SIF). Digestion of purified Car i 1 in SGF and SIF resulted in formation of different digestion-resistant peptides that were capable of binding IgE antibodies from allergic individuals. Digestion stability of Car i 1 and formation of digestion-resistant antigenic peptides may explain why it is a potent sensitising protein in pecan for susceptible individuals. The observation that digestion-resistant peptides are able to bind IgE implies that pecan can trigger systemic allergic reactions even after digestion in the stomach and small intestine.

Original languageEnglish (US)
Pages (from-to)1566-1578
Number of pages13
JournalInternational Journal of Food Science and Technology
Volume53
Issue number6
DOIs
StatePublished - Jun 2018

Fingerprint

Carya
pecans
in vitro digestion
Peptides
albumins
Digestion
Albumins
digestion
peptides
Proteins
Fluids
Railroad cars
Stomach
Allergens
Proteolysis
stomach
hypersensitivity
Immunoglobulin E
Antibodies
Hypersensitivity

Keywords

  • Allergens
  • digestion/digestibility
  • pecans

ASJC Scopus subject areas

  • Food Science
  • Industrial and Manufacturing Engineering

Cite this

In vitro digestion and characterisation of 2S albumin and digestion-resistant peptides in pecan. / Spiric, Jelena; Koppelman, Stef J.; Knulst, Andre; Nordlee, Julie A.; Taylor, Steve L.; Baumert, Joseph L.

In: International Journal of Food Science and Technology, Vol. 53, No. 6, 06.2018, p. 1566-1578.

Research output: Contribution to journalArticle

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