In vitro development of zygotes from prepubertal gilts after microinjection of DNA.

B. L. Williams, A. E. Sparks, R. S. Canseco, J. W. Knight, J. L. Johnson, W. H. Velander, R. L. Page, W. N. Drohan, J. M. Young, R. E. Pearson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and hCG. Zygotes received either pronuclear microinjection of buffer alone, buffer containing a DNA construct, or no microinjection. Zygotes were cultured in vitro in either modified Krebs-Ringer bicarbonate medium (KRB) for 144 h or in mouse oviduct (MO) explant culture with KRB for 48, 72, 96, or 120 h. Pronuclear microinjection of DNA resulted in a lower (P less than .05) cleavage index (CI) than did buffer or no microinjection (CI 2.16 +/- .10 vs 2.80 +/- .13 and 2.93 +/- .10). The CI loss was greatest for DNA-injected zygotes at the two-cell stage of development. Coculture of zygotes in MO resulted in a higher CI (P less than .01) than did culture in KRB. Culture in MO for 72 h was the most beneficial system compared with MO for 48, 96, or 120 h (P less than .05; CI 3.25 +/- .12 vs 2.66 +/- .18, 2.79 +/- .14, and 2.40 +/- .14, respectively). Microinjection of DNA, not merely the mechanical procedure, was detrimental to early zygote development and may be the cause of low pregnancy rates.

Original languageEnglish (US)
Pages (from-to)2207-2211
Number of pages5
JournalJournal of animal science
Volume70
Issue number7
DOIs
StatePublished - Jul 1992

Fingerprint

Zygote
Microinjections
zygote
Oviducts
gilts
oviducts
DNA
Bicarbonates
bicarbonates
mice
Buffers
buffers
Equine Gonadotropins
pregnant mare serum gonadotropin
human chorionic gonadotropin
coculture
Pregnancy Rate
Coculture Techniques
Ovulation
pregnancy rate

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Williams, B. L., Sparks, A. E., Canseco, R. S., Knight, J. W., Johnson, J. L., Velander, W. H., ... Pearson, R. E. (1992). In vitro development of zygotes from prepubertal gilts after microinjection of DNA. Journal of animal science, 70(7), 2207-2211. https://doi.org/10.2527/1992.7072207x

In vitro development of zygotes from prepubertal gilts after microinjection of DNA. / Williams, B. L.; Sparks, A. E.; Canseco, R. S.; Knight, J. W.; Johnson, J. L.; Velander, W. H.; Page, R. L.; Drohan, W. N.; Young, J. M.; Pearson, R. E.

In: Journal of animal science, Vol. 70, No. 7, 07.1992, p. 2207-2211.

Research output: Contribution to journalArticle

Williams, BL, Sparks, AE, Canseco, RS, Knight, JW, Johnson, JL, Velander, WH, Page, RL, Drohan, WN, Young, JM & Pearson, RE 1992, 'In vitro development of zygotes from prepubertal gilts after microinjection of DNA.', Journal of animal science, vol. 70, no. 7, pp. 2207-2211. https://doi.org/10.2527/1992.7072207x
Williams, B. L. ; Sparks, A. E. ; Canseco, R. S. ; Knight, J. W. ; Johnson, J. L. ; Velander, W. H. ; Page, R. L. ; Drohan, W. N. ; Young, J. M. ; Pearson, R. E. / In vitro development of zygotes from prepubertal gilts after microinjection of DNA. In: Journal of animal science. 1992 ; Vol. 70, No. 7. pp. 2207-2211.
@article{8559fc29326b4356a3c378ea3ea78bcf,
title = "In vitro development of zygotes from prepubertal gilts after microinjection of DNA.",
abstract = "The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and hCG. Zygotes received either pronuclear microinjection of buffer alone, buffer containing a DNA construct, or no microinjection. Zygotes were cultured in vitro in either modified Krebs-Ringer bicarbonate medium (KRB) for 144 h or in mouse oviduct (MO) explant culture with KRB for 48, 72, 96, or 120 h. Pronuclear microinjection of DNA resulted in a lower (P less than .05) cleavage index (CI) than did buffer or no microinjection (CI 2.16 +/- .10 vs 2.80 +/- .13 and 2.93 +/- .10). The CI loss was greatest for DNA-injected zygotes at the two-cell stage of development. Coculture of zygotes in MO resulted in a higher CI (P less than .01) than did culture in KRB. Culture in MO for 72 h was the most beneficial system compared with MO for 48, 96, or 120 h (P less than .05; CI 3.25 +/- .12 vs 2.66 +/- .18, 2.79 +/- .14, and 2.40 +/- .14, respectively). Microinjection of DNA, not merely the mechanical procedure, was detrimental to early zygote development and may be the cause of low pregnancy rates.",
author = "Williams, {B. L.} and Sparks, {A. E.} and Canseco, {R. S.} and Knight, {J. W.} and Johnson, {J. L.} and Velander, {W. H.} and Page, {R. L.} and Drohan, {W. N.} and Young, {J. M.} and Pearson, {R. E.}",
year = "1992",
month = "7",
doi = "10.2527/1992.7072207x",
language = "English (US)",
volume = "70",
pages = "2207--2211",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "7",

}

TY - JOUR

T1 - In vitro development of zygotes from prepubertal gilts after microinjection of DNA.

AU - Williams, B. L.

AU - Sparks, A. E.

AU - Canseco, R. S.

AU - Knight, J. W.

AU - Johnson, J. L.

AU - Velander, W. H.

AU - Page, R. L.

AU - Drohan, W. N.

AU - Young, J. M.

AU - Pearson, R. E.

PY - 1992/7

Y1 - 1992/7

N2 - The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and hCG. Zygotes received either pronuclear microinjection of buffer alone, buffer containing a DNA construct, or no microinjection. Zygotes were cultured in vitro in either modified Krebs-Ringer bicarbonate medium (KRB) for 144 h or in mouse oviduct (MO) explant culture with KRB for 48, 72, 96, or 120 h. Pronuclear microinjection of DNA resulted in a lower (P less than .05) cleavage index (CI) than did buffer or no microinjection (CI 2.16 +/- .10 vs 2.80 +/- .13 and 2.93 +/- .10). The CI loss was greatest for DNA-injected zygotes at the two-cell stage of development. Coculture of zygotes in MO resulted in a higher CI (P less than .01) than did culture in KRB. Culture in MO for 72 h was the most beneficial system compared with MO for 48, 96, or 120 h (P less than .05; CI 3.25 +/- .12 vs 2.66 +/- .18, 2.79 +/- .14, and 2.40 +/- .14, respectively). Microinjection of DNA, not merely the mechanical procedure, was detrimental to early zygote development and may be the cause of low pregnancy rates.

AB - The effect of pronuclear microinjection of DNA and culture in excised mouse oviducts on the development of porcine zygotes was assessed in this study. Precocious ovulation was induced in prepubertal gilts with pregnant mare's serum gonadotrophin and hCG. Zygotes received either pronuclear microinjection of buffer alone, buffer containing a DNA construct, or no microinjection. Zygotes were cultured in vitro in either modified Krebs-Ringer bicarbonate medium (KRB) for 144 h or in mouse oviduct (MO) explant culture with KRB for 48, 72, 96, or 120 h. Pronuclear microinjection of DNA resulted in a lower (P less than .05) cleavage index (CI) than did buffer or no microinjection (CI 2.16 +/- .10 vs 2.80 +/- .13 and 2.93 +/- .10). The CI loss was greatest for DNA-injected zygotes at the two-cell stage of development. Coculture of zygotes in MO resulted in a higher CI (P less than .01) than did culture in KRB. Culture in MO for 72 h was the most beneficial system compared with MO for 48, 96, or 120 h (P less than .05; CI 3.25 +/- .12 vs 2.66 +/- .18, 2.79 +/- .14, and 2.40 +/- .14, respectively). Microinjection of DNA, not merely the mechanical procedure, was detrimental to early zygote development and may be the cause of low pregnancy rates.

UR - http://www.scopus.com/inward/record.url?scp=0026887197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026887197&partnerID=8YFLogxK

U2 - 10.2527/1992.7072207x

DO - 10.2527/1992.7072207x

M3 - Article

C2 - 1644695

AN - SCOPUS:0026887197

VL - 70

SP - 2207

EP - 2211

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 7

ER -