In vitro assay for quantitative analysis of axonal regeneration in the spinal cord injury using microfluidic platform

Hyung Joon Kim, Jeong Won Park, Behrad Vahidi, Jae Hwan Byun, Sudong Kim, Qing Ping Hu, Hyun Ryul Ryu, Yekyuong Cho, Noo Li Jeon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper will describe a microfluidic platform that can be used to quantitatively evaluate the regeneration of lesioned axons. The device has two isolated compartments separated by embedded microgrooves and successfully used for long-term culture of primary CNS neurons while isolating the somata in one chamber and directing growth of axons to the other chamber, which is subjected to reproducible axotomy by vacuum aspiration. After 24hours, the lengths of regenerated axons were measured for quantitative analysis and applying inhibitory protein resulted in reduction in length of regenerated axons. Quantification analysis revealed that Nogo and MAG diminished the average length of regenerated axons to 75∼80% of the control.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1919-1921
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - Jan 1 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

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Keywords

  • Axonal regeneration
  • CNS injury
  • Microfluidics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kim, H. J., Park, J. W., Vahidi, B., Byun, J. H., Kim, S., Hu, Q. P., ... Jeon, N. L. (2009). In vitro assay for quantitative analysis of axonal regeneration in the spinal cord injury using microfluidic platform. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1919-1921). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.