In vitro and in vivo protective effects of eliprodil in the retina

M. L. Chandler, I. H. Pang, R. Doshi, E. M. Wexler, R. J. Walters, Scott A Nawy, L. Desantis, M. A. Kapin

Research output: Contribution to journalArticle

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Abstract

Purpose. Evaluate the protection of eliprodil, an NMDA-receptor polyamine-site antagonist, against excitatory amino acid- or ischemia-induced insults on retinal cells and retina in vivo. Methods, Neonatal rat retinal ganglion cells (RGC) were used in whole-cell recording and survival (assayed by counting Di-I-labelled cells) studies. In in vivo experiments, retinal ischemia, as assessed by a cessation of ERG activity, was effected in rabbits by raising intraocular pressure. Excitotoxicity was produced by intravitreal injection of NMDA (20 nmol in 5 /xl), which caused a decrease of cells in the ganglion cell layer (GCL) and retinal choline acetyltransferase activity (ChAT). Results. Eliprodil dose-dependently suppressed NMDA (200 /iM)-activated currents in RGC, and prevented cell death induced by 100 /*M glutamate with an ECSO of 1 nM. At 100 nM of eliprodil, 100% of cells survived the glutamate insult; however, eliprodil did not prevent anoxia-induced in vitro RGC cell death. In the anesthetized rabbit, retinal ischemia resulted in a 60-70% depression in ERG a- and b-waves. Eliprodil (1-10 mg/kg, i.p.) administered prior to ischemia, in a dose-dependent manner, attenuated the depressed ERG throughout a 48 h period. At. 10 mg/kg, i.p., eliprodil also completely prevented the loss of retinal ChAT and loss of cells in the GCL. Conclusions. Eliprodil is protective against cytotoxic insults due to ischemia or excitatory amino acids in retinal tissues and cells.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997

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Retina
Retinal Ganglion Cells
Ischemia
Choline O-Acetyltransferase
N-Methylaspartate
Glutamic Acid
Cell Death
Rabbits
Excitatory Amino Acid Antagonists
Excitatory Amino Acids
Intravitreal Injections
Polyamines
Patch-Clamp Techniques
In Vitro Techniques
eliprodil
N-Methyl-D-Aspartate Receptors
Intraocular Pressure
Ganglia
Cell Survival

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Chandler, M. L., Pang, I. H., Doshi, R., Wexler, E. M., Walters, R. J., Nawy, S. A., ... Kapin, M. A. (1997). In vitro and in vivo protective effects of eliprodil in the retina. Investigative Ophthalmology and Visual Science, 38(4).

In vitro and in vivo protective effects of eliprodil in the retina. / Chandler, M. L.; Pang, I. H.; Doshi, R.; Wexler, E. M.; Walters, R. J.; Nawy, Scott A; Desantis, L.; Kapin, M. A.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 01.12.1997.

Research output: Contribution to journalArticle

Chandler, ML, Pang, IH, Doshi, R, Wexler, EM, Walters, RJ, Nawy, SA, Desantis, L & Kapin, MA 1997, 'In vitro and in vivo protective effects of eliprodil in the retina', Investigative Ophthalmology and Visual Science, vol. 38, no. 4.
Chandler ML, Pang IH, Doshi R, Wexler EM, Walters RJ, Nawy SA et al. In vitro and in vivo protective effects of eliprodil in the retina. Investigative Ophthalmology and Visual Science. 1997 Dec 1;38(4).
Chandler, M. L. ; Pang, I. H. ; Doshi, R. ; Wexler, E. M. ; Walters, R. J. ; Nawy, Scott A ; Desantis, L. ; Kapin, M. A. / In vitro and in vivo protective effects of eliprodil in the retina. In: Investigative Ophthalmology and Visual Science. 1997 ; Vol. 38, No. 4.
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abstract = "Purpose. Evaluate the protection of eliprodil, an NMDA-receptor polyamine-site antagonist, against excitatory amino acid- or ischemia-induced insults on retinal cells and retina in vivo. Methods, Neonatal rat retinal ganglion cells (RGC) were used in whole-cell recording and survival (assayed by counting Di-I-labelled cells) studies. In in vivo experiments, retinal ischemia, as assessed by a cessation of ERG activity, was effected in rabbits by raising intraocular pressure. Excitotoxicity was produced by intravitreal injection of NMDA (20 nmol in 5 /xl), which caused a decrease of cells in the ganglion cell layer (GCL) and retinal choline acetyltransferase activity (ChAT). Results. Eliprodil dose-dependently suppressed NMDA (200 /iM)-activated currents in RGC, and prevented cell death induced by 100 /*M glutamate with an ECSO of 1 nM. At 100 nM of eliprodil, 100{\%} of cells survived the glutamate insult; however, eliprodil did not prevent anoxia-induced in vitro RGC cell death. In the anesthetized rabbit, retinal ischemia resulted in a 60-70{\%} depression in ERG a- and b-waves. Eliprodil (1-10 mg/kg, i.p.) administered prior to ischemia, in a dose-dependent manner, attenuated the depressed ERG throughout a 48 h period. At. 10 mg/kg, i.p., eliprodil also completely prevented the loss of retinal ChAT and loss of cells in the GCL. Conclusions. Eliprodil is protective against cytotoxic insults due to ischemia or excitatory amino acids in retinal tissues and cells.",
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AU - Pang, I. H.

AU - Doshi, R.

AU - Wexler, E. M.

AU - Walters, R. J.

AU - Nawy, Scott A

AU - Desantis, L.

AU - Kapin, M. A.

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N2 - Purpose. Evaluate the protection of eliprodil, an NMDA-receptor polyamine-site antagonist, against excitatory amino acid- or ischemia-induced insults on retinal cells and retina in vivo. Methods, Neonatal rat retinal ganglion cells (RGC) were used in whole-cell recording and survival (assayed by counting Di-I-labelled cells) studies. In in vivo experiments, retinal ischemia, as assessed by a cessation of ERG activity, was effected in rabbits by raising intraocular pressure. Excitotoxicity was produced by intravitreal injection of NMDA (20 nmol in 5 /xl), which caused a decrease of cells in the ganglion cell layer (GCL) and retinal choline acetyltransferase activity (ChAT). Results. Eliprodil dose-dependently suppressed NMDA (200 /iM)-activated currents in RGC, and prevented cell death induced by 100 /*M glutamate with an ECSO of 1 nM. At 100 nM of eliprodil, 100% of cells survived the glutamate insult; however, eliprodil did not prevent anoxia-induced in vitro RGC cell death. In the anesthetized rabbit, retinal ischemia resulted in a 60-70% depression in ERG a- and b-waves. Eliprodil (1-10 mg/kg, i.p.) administered prior to ischemia, in a dose-dependent manner, attenuated the depressed ERG throughout a 48 h period. At. 10 mg/kg, i.p., eliprodil also completely prevented the loss of retinal ChAT and loss of cells in the GCL. Conclusions. Eliprodil is protective against cytotoxic insults due to ischemia or excitatory amino acids in retinal tissues and cells.

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