In vitro activities of parenteral β-lactam antimicrobials against TEM-10, TEM-26- and SHV-5-derived extended-spectrum β-lactamases expressed in an isogenic Escherichia coli host

Jill A. Rebuck, Keith M. Olsen, Paul D Fey, Kimberly L. Bergman, Mark Edmund Rupp

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12 Citations (Scopus)

Abstract

The in vitro activities were determined and time-kill studies of cefepime, imipenem-cilastatin, meropenem and piperacillin-tazobactam were performed against SHV- and TEM-derived extended-spectrum β-lactamases (ESBLs). Sequence-confirmed SHV-5, TEM-10 and TEM-26 β-lactamases were transferred into Escherichia coli C600N by conjugation. Imipenem and meropenem were more active (MIC range 0.0625-0.25 mg/L) than cefepime (MIC range 2-8 mg/L) and piperacillin-tazobactam (MIC range 8-32 mg/L). Regrowth of strains expressing TEM-10 and TEM-26 was noted at all cefepime and piperacillin-tazobactam concentrations studied. Imipenem-cilastatin and meropenem demonstrated rapid, sustained bactericidal activity uninfluenced by the type of ESBL expressed.

Original languageEnglish (US)
Pages (from-to)461-464
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume46
Issue number3
StatePublished - Oct 2 2000

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meropenem
Lactams
Escherichia coli
Imipenem
cefepime
In Vitro Techniques
tazobactam drug combination piperacillin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

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title = "In vitro activities of parenteral β-lactam antimicrobials against TEM-10, TEM-26- and SHV-5-derived extended-spectrum β-lactamases expressed in an isogenic Escherichia coli host",
abstract = "The in vitro activities were determined and time-kill studies of cefepime, imipenem-cilastatin, meropenem and piperacillin-tazobactam were performed against SHV- and TEM-derived extended-spectrum β-lactamases (ESBLs). Sequence-confirmed SHV-5, TEM-10 and TEM-26 β-lactamases were transferred into Escherichia coli C600N by conjugation. Imipenem and meropenem were more active (MIC range 0.0625-0.25 mg/L) than cefepime (MIC range 2-8 mg/L) and piperacillin-tazobactam (MIC range 8-32 mg/L). Regrowth of strains expressing TEM-10 and TEM-26 was noted at all cefepime and piperacillin-tazobactam concentrations studied. Imipenem-cilastatin and meropenem demonstrated rapid, sustained bactericidal activity uninfluenced by the type of ESBL expressed.",
author = "Rebuck, {Jill A.} and Olsen, {Keith M.} and Fey, {Paul D} and Bergman, {Kimberly L.} and Rupp, {Mark Edmund}",
year = "2000",
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language = "English (US)",
volume = "46",
pages = "461--464",
journal = "Journal of Antimicrobial Chemotherapy",
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TY - JOUR

T1 - In vitro activities of parenteral β-lactam antimicrobials against TEM-10, TEM-26- and SHV-5-derived extended-spectrum β-lactamases expressed in an isogenic Escherichia coli host

AU - Rebuck, Jill A.

AU - Olsen, Keith M.

AU - Fey, Paul D

AU - Bergman, Kimberly L.

AU - Rupp, Mark Edmund

PY - 2000/10/2

Y1 - 2000/10/2

N2 - The in vitro activities were determined and time-kill studies of cefepime, imipenem-cilastatin, meropenem and piperacillin-tazobactam were performed against SHV- and TEM-derived extended-spectrum β-lactamases (ESBLs). Sequence-confirmed SHV-5, TEM-10 and TEM-26 β-lactamases were transferred into Escherichia coli C600N by conjugation. Imipenem and meropenem were more active (MIC range 0.0625-0.25 mg/L) than cefepime (MIC range 2-8 mg/L) and piperacillin-tazobactam (MIC range 8-32 mg/L). Regrowth of strains expressing TEM-10 and TEM-26 was noted at all cefepime and piperacillin-tazobactam concentrations studied. Imipenem-cilastatin and meropenem demonstrated rapid, sustained bactericidal activity uninfluenced by the type of ESBL expressed.

AB - The in vitro activities were determined and time-kill studies of cefepime, imipenem-cilastatin, meropenem and piperacillin-tazobactam were performed against SHV- and TEM-derived extended-spectrum β-lactamases (ESBLs). Sequence-confirmed SHV-5, TEM-10 and TEM-26 β-lactamases were transferred into Escherichia coli C600N by conjugation. Imipenem and meropenem were more active (MIC range 0.0625-0.25 mg/L) than cefepime (MIC range 2-8 mg/L) and piperacillin-tazobactam (MIC range 8-32 mg/L). Regrowth of strains expressing TEM-10 and TEM-26 was noted at all cefepime and piperacillin-tazobactam concentrations studied. Imipenem-cilastatin and meropenem demonstrated rapid, sustained bactericidal activity uninfluenced by the type of ESBL expressed.

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