In situ visualization of dynamic interactions of cellulase and cellulose molecules

Bo Song, Ning Xi, Ruiguo Yang, Zhiyong Sun, Liangliang Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Enzymatic hydrolysis of cellulose from biomass is potentially considered as a new approach for the next generation of biofuels for sustainable energy. However, because of the rigid and complex structure of cellulose, the low efficiency of the enzymatic hydrolysis has been deemed as the bottleneck in biorefining industry. The mechanism of enzymatic hydrolysis of cellulose is not well understood due to the absence of direct observation methods. In the present study, in order to study the mechanism of enzymatic hydrolysis of cellulose, we used an atomic force microscopy (AFM) to dynamically observe the changes happening during the cellulase and cellulose interaction. To improve the imaging rate of AFM (which was too slow to capture the interactions between these two nano structures), a scan strategy named compressive scan is used, associated with prior knowledge based image reconstruction methods. With the help of compressive scan, we are able to dynamically capture the action of the cellulase-CBH I (from family 7 cellobiohydrolase) on the cellulose crystal nanofibrils. The result is helpful for understanding the mechanism of enzymatic interaction and increasing the efficiency of hydrolysis process.

Original languageEnglish (US)
Title of host publication14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages13-17
Number of pages5
ISBN (Electronic)9781479956227
DOIs
StatePublished - Nov 26 2014
Event2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 - Toronto, Canada
Duration: Aug 18 2014Aug 21 2014

Publication series

Name14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014

Other

Other2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
CountryCanada
CityToronto
Period8/18/148/21/14

Fingerprint

Cellulose
cellulose
Enzymatic hydrolysis
Visualization
Molecules
hydrolysis
Interaction
molecules
Atomic Force Microscopy
interactions
Atomic force microscopy
atomic force microscopy
Image Reconstruction
Biofuels
Biomass
rigid structures
biomass
Knowledge-based
image reconstruction
Image reconstruction

Keywords

  • Atomic force microscopy
  • Cellulase
  • Cellulose
  • Enzymatic hydrolysis
  • Fast scan
  • In situ visualization

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Computer Science Applications
  • Modeling and Simulation
  • Instrumentation

Cite this

Song, B., Xi, N., Yang, R., Sun, Z., & Chen, L. (2014). In situ visualization of dynamic interactions of cellulase and cellulose molecules. In 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014 (pp. 13-17). [6968134] (14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANO.2014.6968134

In situ visualization of dynamic interactions of cellulase and cellulose molecules. / Song, Bo; Xi, Ning; Yang, Ruiguo; Sun, Zhiyong; Chen, Liangliang.

14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014. Institute of Electrical and Electronics Engineers Inc., 2014. p. 13-17 6968134 (14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Song, B, Xi, N, Yang, R, Sun, Z & Chen, L 2014, In situ visualization of dynamic interactions of cellulase and cellulose molecules. in 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014., 6968134, 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014, Institute of Electrical and Electronics Engineers Inc., pp. 13-17, 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014, Toronto, Canada, 8/18/14. https://doi.org/10.1109/NANO.2014.6968134
Song B, Xi N, Yang R, Sun Z, Chen L. In situ visualization of dynamic interactions of cellulase and cellulose molecules. In 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014. Institute of Electrical and Electronics Engineers Inc. 2014. p. 13-17. 6968134. (14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014). https://doi.org/10.1109/NANO.2014.6968134
Song, Bo ; Xi, Ning ; Yang, Ruiguo ; Sun, Zhiyong ; Chen, Liangliang. / In situ visualization of dynamic interactions of cellulase and cellulose molecules. 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 13-17 (14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014).
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