In situ hybridization for the detection and localization of swine Chlamydia trachomatis

C. Chae, D. S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, Douglas G Rogers, K. D.E. Everett, A. A. Andersen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally with swine C. trachmatis strain R27. Archived formalin-fixed, paraffin-embedded tissues from piglets euthanatized 4-7 days postinoculation were examined by in situ hybridization for C. trachomatis nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA or omp l mRNA molecules of the swine C. trachomatis strains. Positive hybridization signals were detected in bronchial epithelial cells, bronchiolar epithelial cells, pneumocytes, alveolar and interstitial macrophages, and jejunal and ileal enterocytes. Chlamydia-infected cells had a strong signal that was confined to the intracytoplasmic inclusions. Positive hybridization signals were not detected in tissue sections from an uninfected control piglet or in C. psittaci-infected sheep placenta. The morphology of host cells was preserved despite the relatively high temperature required in parts of the incubation procedure. The data indicate that in situ hybridization can be used to detect swine C. trachomatis in formalin-fixed, paraffin-embedded tissue specimens.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalVeterinary pathology
Volume36
Issue number2
DOIs
StatePublished - Jan 1 1999

Fingerprint

Chlamydia trachomatis
in situ hybridization
In Situ Hybridization
Swine
piglets
swine
formalin
Paraffin
alkanes
Formaldehyde
epithelial cells
hybridization
Epithelial Cells
pneumocytes
Germ-Free Life
Alveolar Epithelial Cells
Digoxigenin
digoxigenin
Chlamydia
Ribosomal RNA

Keywords

  • Chlamydia trachomatis
  • Enteritis
  • In situ hybridization
  • Pneumonia
  • Swine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Chae, C., Cheon, D. S., Kwon, D., Kim, O., Kim, B., Suh, J., ... Andersen, A. A. (1999). In situ hybridization for the detection and localization of swine Chlamydia trachomatis. Veterinary pathology, 36(2), 133-137. https://doi.org/10.1354/vp.36-2-133

In situ hybridization for the detection and localization of swine Chlamydia trachomatis. / Chae, C.; Cheon, D. S.; Kwon, D.; Kim, O.; Kim, B.; Suh, J.; Rogers, Douglas G; Everett, K. D.E.; Andersen, A. A.

In: Veterinary pathology, Vol. 36, No. 2, 01.01.1999, p. 133-137.

Research output: Contribution to journalArticle

Chae, C, Cheon, DS, Kwon, D, Kim, O, Kim, B, Suh, J, Rogers, DG, Everett, KDE & Andersen, AA 1999, 'In situ hybridization for the detection and localization of swine Chlamydia trachomatis', Veterinary pathology, vol. 36, no. 2, pp. 133-137. https://doi.org/10.1354/vp.36-2-133
Chae, C. ; Cheon, D. S. ; Kwon, D. ; Kim, O. ; Kim, B. ; Suh, J. ; Rogers, Douglas G ; Everett, K. D.E. ; Andersen, A. A. / In situ hybridization for the detection and localization of swine Chlamydia trachomatis. In: Veterinary pathology. 1999 ; Vol. 36, No. 2. pp. 133-137.
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