In situ enzymatic screening (ISES)

A tool for catalyst discovery and reaction development

David B Berkowitz, Mohua Bose, Sungjo Choi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Can enzymes help organic chemists identify new transition-metal catalysts? The first example of the in situ enzymatic monitoring of an organic transformation is described. Thus, a transition-metal-mediated allylic-amination reaction in the organic layer (see scheme; R=para-methoxyphenyl, tosyl) leads to release of ethanol which causes the spectroscopically observable formation of NADH in the aqueous phase, This approach uncovers a new Ni0-promoted route to β,γ-unsaturated amino acids, HMDS = 1,1,1,3,3,3-hexamethyldisilazane.

Original languageEnglish (US)
Pages (from-to)1603-1607
Number of pages5
JournalAngewandte Chemie - International Edition
Volume41
Issue number9
DOIs
StatePublished - May 3 2002

Fingerprint

Transition metals
Screening
Amination
Catalysts
NAD
Amino acids
Ethanol
Enzymes
Amino Acids
Monitoring
hexamethylsilazane

Keywords

  • Allylic substitution
  • Amino acids
  • Catalyst screening
  • Enzymes
  • Nickel
  • Transition metals

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

In situ enzymatic screening (ISES) : A tool for catalyst discovery and reaction development. / Berkowitz, David B; Bose, Mohua; Choi, Sungjo.

In: Angewandte Chemie - International Edition, Vol. 41, No. 9, 03.05.2002, p. 1603-1607.

Research output: Contribution to journalArticle

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