In situ enzymatic screening (ISES): A tool for catalyst discovery and reaction development

David B Berkowitz, Mohua Bose, Sungjo Choi

Research output: Contribution to journalArticle

36 Scopus citations


Can enzymes help organic chemists identify new transition-metal catalysts? The first example of the in situ enzymatic monitoring of an organic transformation is described. Thus, a transition-metal-mediated allylic-amination reaction in the organic layer (see scheme; R=para-methoxyphenyl, tosyl) leads to release of ethanol which causes the spectroscopically observable formation of NADH in the aqueous phase, This approach uncovers a new Ni0-promoted route to β,γ-unsaturated amino acids, HMDS = 1,1,1,3,3,3-hexamethyldisilazane.

Original languageEnglish (US)
Pages (from-to)1603-1607
Number of pages5
JournalAngewandte Chemie - International Edition
Issue number9
Publication statusPublished - May 3 2002



  • Allylic substitution
  • Amino acids
  • Catalyst screening
  • Enzymes
  • Nickel
  • Transition metals

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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