In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films

Yi Zou, Lei Wan, Jenifer Blacklock, David Oupicky, Guangzhao Mao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Synthetic vector-based gene delivery systems continue to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nano-systems. Here we describe atomic force microscope application to the understanding of DNA release dynamics from bioreducible polycation-based nano-systems. The two nano-systems are polyplex nanoparticles and layer-by-layer fi lms.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages183-193
Number of pages11
Volume948
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume948
ISSN (Print)10643745

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Keywords

  • DNA release
  • Gene delivery
  • In situ AFM
  • Layer-by-layer film
  • Polyplex

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Zou, Y., Wan, L., Blacklock, J., Oupicky, D., & Mao, G. (2013). In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films. In Methods in Molecular Biology (Vol. 948, pp. 183-193). (Methods in Molecular Biology; Vol. 948). https://doi.org/10.1007/978-1-62703-140-0-13