In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films

Yi Zou, Lei Wan, Jenifer Blacklock, David Oupicky, Guangzhao Mao

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Synthetic vector-based gene delivery systems continue to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nano-systems. Here we describe atomic force microscope application to the understanding of DNA release dynamics from bioreducible polycation-based nano-systems. The two nano-systems are polyplex nanoparticles and layer-by-layer fi lms.

Original languageEnglish (US)
Title of host publicationNanotechnology for Nucleic Acid Delivery
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages183-193
Number of pages11
ISBN (Print)9781627031394
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume948
ISSN (Print)1064-3745

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Keywords

  • DNA release
  • Gene delivery
  • In situ AFM
  • Layer-by-layer film
  • Polyplex

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zou, Y., Wan, L., Blacklock, J., Oupicky, D., & Mao, G. (2013). In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols (pp. 183-193). (Methods in Molecular Biology; Vol. 948). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-140-0_13