In situ activated T lymphocytes in active versus stable periodontal lesions

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Abstract

A double labeling technique was developed to detect T cell phenotype and HLA‐DR (activation) antigens on infiltrating cells in gingival biopsy sites classified as either periodontally active (≥ mm attachment loss within past 3 months), clinically similar but stable, or healthy. Serial cryostat sections were obtained from each of the above disease category biopsies in 13 periodontal maintenance patients, and were processed with sequential Leu series (T cell subsets) avidinbiotin‐peroxidase followed by anti‐HLA‐DR indirect alkaline phosphatase labeling protocols. Labeled cells were counted in repeatable fields in the sulcular, middle and oral thirds of each section, and HLA‐DR+ T cells (activated) were calculated. Total HLA‐DR‐labeled cells and HLA‐DR‐positive pan‐T cells and T helper cells (Th) were all more numerous in active specimens than in healthy sites (p<0.05), and in sulcular fields than in the oral third of the section (p<0.05). Although activated T suppressor cell (T5) densities did not vary statistically according to tissue location, active biopsies had more cells per field than either stable or healthy specimens (p<0.05), especially in the sulcular third. A majority of pan‐T, Ts and Th cells appeared to display activation markers in active, stable and healthy biopsies. This study supports the functional role of T lymphocytes in modulating the immune response in periodontal tissues.

Original languageEnglish (US)
Pages (from-to)295-302
Number of pages8
JournalJournal of Periodontal Research
Volume23
Issue number5
DOIs
StatePublished - Jan 1 1988

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T-Lymphocytes
Biopsy
Helper-Inducer T-Lymphocytes
T-Lymphocyte Subsets
Alkaline Phosphatase
Cell Count
Phenotype
Antigens

ASJC Scopus subject areas

  • Periodontics

Cite this

In situ activated T lymphocytes in active versus stable periodontal lesions. / Reinhardt, Richard A; McDonald, Thomas L; Bolton, R. W.; DuBois, L. M.; Feely, Dennis E; Kaldahl, Wayne Berwyn.

In: Journal of Periodontal Research, Vol. 23, No. 5, 01.01.1988, p. 295-302.

Research output: Contribution to journalArticle

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