Impulsive pressurization of neuronal cells for traumatic brain injury study.

Matthew Nienaber, Jeong Soon Lee, Ruqiang Feng, Jung Yul Lim

Research output: Contribution to journalArticle

Abstract

A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injury (TBI). We demonstrate in this video article how blast TBI-relevant impulsive pressurization is applied to the neuronal cells in vitro. This is achieved by using well-controlled pressure pulse created by a specialized Kolsky bar device, with complete pressure history within the cell pressurization chamber recorded. Pressurized neuronal cells are inspected immediately after pressurization, or further incubated to examine the long-term effects of impulsive pressurization on neurite/axonal outgrowth, neuronal gene expression, apoptosis, etc. We observed that impulsive pressurization at about 2 MPa induces distinct neurite loss relative to unpressurized cells. Our technique provides a novel method to investigate the molecular/cellular mechanisms of blast TBI, via impulsive pressurization of brain cells at well-controlled pressure magnitude and duration.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number56
StatePublished - 2011
Externally publishedYes

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Pressurization
Brain
Pressure
Equipment and Supplies
Neurites
Cell death
Traumatic Brain Injury
Gene expression
Apoptosis
Blood Pressure
Gene Expression
History

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Impulsive pressurization of neuronal cells for traumatic brain injury study. / Nienaber, Matthew; Lee, Jeong Soon; Feng, Ruqiang; Lim, Jung Yul.

In: Journal of visualized experiments : JoVE, No. 56, 2011.

Research output: Contribution to journalArticle

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