Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.

Joelle Wellemans, Mathai George, Ronald L. Cerny, Michael L. Gross

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalPolycyclic Aromatic Compounds
Volume6
Issue number1-4
DOIs
StatePublished - Jan 1994

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Keywords

  • PAH-DNA adducts
  • Tandem MS
  • array detector
  • coaxial cf-FAB
  • derivatization
  • detection limit

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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