Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.

Joelle Wellemans, Mathai George, Ronald Cerny, Michael L. Gross

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalPolycyclic Aromatic Compounds
Volume6
Issue number1-4
DOIs
StatePublished - Jan 1 1994

Fingerprint

Adenine
Polycyclic aromatic hydrocarbons
Mass spectrometry
Guanine
Derivatives
Atoms
polycyclic aromatic hydrocarbons-DNA adduct
DNA Adducts

Keywords

  • PAH-DNA adducts
  • Tandem MS
  • array detector
  • coaxial cf-FAB
  • derivatization
  • detection limit

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts. / Wellemans, Joelle; George, Mathai; Cerny, Ronald; Gross, Michael L.

In: Polycyclic Aromatic Compounds, Vol. 6, No. 1-4, 01.01.1994, p. 103-110.

Research output: Contribution to journalArticle

Wellemans, Joelle ; George, Mathai ; Cerny, Ronald ; Gross, Michael L. / Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts. In: Polycyclic Aromatic Compounds. 1994 ; Vol. 6, No. 1-4. pp. 103-110.
@article{ded41d93006a48f1967c77287122f4e1,
title = "Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.",
abstract = "The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.",
keywords = "PAH-DNA adducts, Tandem MS, array detector, coaxial cf-FAB, derivatization, detection limit",
author = "Joelle Wellemans and Mathai George and Ronald Cerny and Gross, {Michael L.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1080/10406639408031173",
language = "English (US)",
volume = "6",
pages = "103--110",
journal = "Polycyclic Aromatic Compounds",
issn = "1040-6638",
publisher = "Taylor and Francis Ltd.",
number = "1-4",

}

TY - JOUR

T1 - Improving the Sensitivity of FAB Tandem Mass Spectrometry for PAH-DNA Adducts.

AU - Wellemans, Joelle

AU - George, Mathai

AU - Cerny, Ronald

AU - Gross, Michael L.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.

AB - The mass spectrometric detection limits for guanine, adenine, and an adenine/dimethylbenzanthracene adduct were greatly improved by converting the materials to pentafluorobenzyl derivatives, as well as by doing coaxial continuous flow-FAB (coaxial cf-Fast Atom Bombardment). The lowest detection limit (approximately 10 pg) was achieved when the derivatized sample was introduced by coaxial cf-FAB and the determination was by full-scan mass spectrometry. The lowest detection limit achieved by tandem mass spectrometry was 50 pg obtained after derivatization, coaxial cf-FAB introduction and detection with an extended mass range array.

KW - PAH-DNA adducts

KW - Tandem MS

KW - array detector

KW - coaxial cf-FAB

KW - derivatization

KW - detection limit

UR - http://www.scopus.com/inward/record.url?scp=8944249450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8944249450&partnerID=8YFLogxK

U2 - 10.1080/10406639408031173

DO - 10.1080/10406639408031173

M3 - Article

VL - 6

SP - 103

EP - 110

JO - Polycyclic Aromatic Compounds

JF - Polycyclic Aromatic Compounds

SN - 1040-6638

IS - 1-4

ER -