Improving baculovirus resistance to UV inactivation

Increased virulence resulting from expression of a DNA repair enzyme

Dustin T. Petrik, Angela Iseli, Beth A. Montelone, James L Van Etten, Rollie J. Clem

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The use of baculoviruses as biological control agents is hampered by their susceptibility to inactivation by ultraviolet (UV) light. In an attempt to reduce UV inactivation, an algal virus pyrimidine dimer-specific glycosylase, cv-PDG, was expressed in the baculovirus Autographa californica M nucleopolyhedrovirus (AcMNPV), and the infectivity of recombinant viruses expressing cv-PDG was measured after exposure to UV light. Expression of cv-PDG resulted in a 3-fold decrease in inactivation of budded virus by UV as measured by plaque assay in Spodoptera frugiperda Sf21 cells. However, occluded viruses expressing cv-PDG were not more resistant to UV inactivation than wild type AcMNPV when fed to either S. frugiperda or Trichoplusia ni neonate larvae. Surprisingly, however, viruses expressing cv-PDG showed a significant decrease in both the dose of occluded virus required for oral lethality and the time required for lethality compared to control virus, but these effects were only seen in S. frugiperda and not in T. ni larvae.

Original languageEnglish (US)
Pages (from-to)50-56
Number of pages7
JournalJournal of Invertebrate Pathology
Volume82
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Baculoviridae
DNA repair
virulence
repair
virus
inactivation
enzyme
DNA
viruses
enzymes
Spodoptera frugiperda
Autographa californica multiple nucleopolyhedrovirus
Trichoplusia ni
ultraviolet radiation
glycosylases
larva
infectivity
larvae
neonate
pyrimidines

Keywords

  • Autographa californica M nucleopolyhedrovirus
  • Baculovirus
  • Biological control
  • Pyrimidine dimer-specific glycosylase
  • Spodoptera frugiperda
  • Trichoplusia ni
  • Ultraviolet light

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Improving baculovirus resistance to UV inactivation : Increased virulence resulting from expression of a DNA repair enzyme. / Petrik, Dustin T.; Iseli, Angela; Montelone, Beth A.; Van Etten, James L; Clem, Rollie J.

In: Journal of Invertebrate Pathology, Vol. 82, No. 1, 01.01.2003, p. 50-56.

Research output: Contribution to journalArticle

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