Improved Rapid Methodology for the Isolation of Nucleic Acids from Agarose Gels

Steven Tracy

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Improved methodology is presented with which DNA may be rapidly isolated from agarose gels. Hydroxyapatite is used to bind the nucleic acid after agarose solubilization and a sodium citrate buffer is used to elute the nucleic acid free of agarose. Rapid concentration of the sample may then be effected by ethanol precipitation. Purified oyster glycogen may be used as carrier in this regard and does not inhibit restriction endonucle-ases nor T4 DNA ligase in the concentrations used. This methodology is useful for the isolation of single- and double-stranded DNA, supercoil plasmid DNA, and mRNA.

Original languageEnglish (US)
Pages (from-to)251-268
Number of pages18
JournalPreparative Biochemistry
Volume11
Issue number3
DOIs
StatePublished - Jan 1 1981

Fingerprint

Sepharose
Nucleic Acids
Gels
DNA
DNA Ligases
Ostreidae
Single-Stranded DNA
Durapatite
Glycogen
Buffers
Plasmids
Ethanol
Messenger RNA
sodium citrate

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Improved Rapid Methodology for the Isolation of Nucleic Acids from Agarose Gels. / Tracy, Steven.

In: Preparative Biochemistry, Vol. 11, No. 3, 01.01.1981, p. 251-268.

Research output: Contribution to journalArticle

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