Impairment of synaptic transmission by transient hypoxia in hippocampal slices

Improved recovery in glutathione peroxidase transgenic mice

Denis Furling, Ghribi Othman, Ahmed Lahsaini, Marc Edouard Mirault, Guy Massicotte

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

There is increasing evidence that oxygen free radicals contribute to ischemic brain injury. It is unclear, however, to what extent specific antioxidant enzymes can prevent or reverse the impairment of synaptic function caused by transient hypoxia. In this study, we investigated in transgenic (Tg) mice whether a moderate increase in glutathione peroxidase-1 (GPx1) may improve the capacity of CA1 pyramidal cells to recover synaptic transmission after a short period of hypoxia in vitro. In control hippocampal slices, transient hypoxia (7-9 min) produced irreversible loss of excitatory postsynaptic potentials. Complete recovery of synaptic transmission was observed with homozygous Tg-MT-GPx-6 mice after reoxygenation, and, after repeated episodes of hypoxia, synaptic transmission was still viable in most Tg slices, in contrast to non-Tg slices. Moreover, hypoxic episodes abolished the capacity of hippocampal slices to generate long-term potentiation in area CA1 of control mice, whereas a significant extent of long-term potentiation expression was still preserved in Tg tissues. We also demonstrated that susceptibility to N-methyl-D-aspartate-mediated oxidative injury was reduced in Tg hippocampal slices. In conclusion, our results suggest that a moderate GPx increase can be sufficient to prevent irreversible functional damage produced by transient hypoxia in the hippocampus and to help maintain basic electrophysiological mechanisms involved in memory formation.

Original languageEnglish (US)
Pages (from-to)4351-4356
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number8
DOIs
StatePublished - Apr 11 2000

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Glutathione Peroxidase
Synaptic Transmission
Transgenic Mice
Long-Term Potentiation
Pyramidal Cells
Excitatory Postsynaptic Potentials
N-Methylaspartate
Brain Injuries
Free Radicals
Reactive Oxygen Species
Hippocampus
Antioxidants
Hypoxia
Wounds and Injuries
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Impairment of synaptic transmission by transient hypoxia in hippocampal slices : Improved recovery in glutathione peroxidase transgenic mice. / Furling, Denis; Othman, Ghribi; Lahsaini, Ahmed; Mirault, Marc Edouard; Massicotte, Guy.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 8, 11.04.2000, p. 4351-4356.

Research output: Contribution to journalArticle

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