Impairment of glycoprotein secretion by phenobarbital in rat liver slices

Dean J. Tuma, Richard B. Jennett, Michael F. Sorrell

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effects of phenobarbital on protein and glycoprotein synthesis and secretion were studied in rat liver slices. Phenobarbital (2 mM) decreased [14C]-glucosamine and [14C]leucine incorporation into liver proteins and markedly inhibited their incorporation into medium (secretory) proteins. This inhibitory effect of phenobarbital was dose dependent and not reversible under the conditions of this study. In the presence of cycloheximide, an inhibitor of peptide synthesis, phenobarbital still inhibited the release of glycoproteins into the medium; however, the specific activity of liver glycoproteins was increased. The effects of phenobarbital on hepatic macromolecular secretion, independent of its effects on synthesis, were determined by prelabeling proteins in a liver slice system with either [14C]leucine of [14C]glucosamine. When phenobarbital was present, the secretion of these prelabeled proteins into the medium was impaired. 12 h after intraperitoneal injections of phenobarbital, glycoprotein secretion was inhibited from liver slices prepared from the pretreated rats. This inhibition of secretion occurred even though protein synthesis was stimulated and intracellular glycosylations unaffected. The results of this study indicate that phenobarbital impairs the secretion of glycoproteins by the liver.

Original languageEnglish (US)
Pages (from-to)144-152
Number of pages9
JournalBBA - General Subjects
Volume544
Issue number1
DOIs
StatePublished - Nov 15 1978

Fingerprint

Phenobarbital
Liver
Rats
Glycoproteins
Proteins
Glucosamine
Leucine
Glycosylation
Cycloheximide
Intraperitoneal Injections
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

Impairment of glycoprotein secretion by phenobarbital in rat liver slices. / Tuma, Dean J.; Jennett, Richard B.; Sorrell, Michael F.

In: BBA - General Subjects, Vol. 544, No. 1, 15.11.1978, p. 144-152.

Research output: Contribution to journalArticle

Tuma, Dean J. ; Jennett, Richard B. ; Sorrell, Michael F. / Impairment of glycoprotein secretion by phenobarbital in rat liver slices. In: BBA - General Subjects. 1978 ; Vol. 544, No. 1. pp. 144-152.
@article{7addd90f25474b73ae948e0c6bf70249,
title = "Impairment of glycoprotein secretion by phenobarbital in rat liver slices",
abstract = "The effects of phenobarbital on protein and glycoprotein synthesis and secretion were studied in rat liver slices. Phenobarbital (2 mM) decreased [14C]-glucosamine and [14C]leucine incorporation into liver proteins and markedly inhibited their incorporation into medium (secretory) proteins. This inhibitory effect of phenobarbital was dose dependent and not reversible under the conditions of this study. In the presence of cycloheximide, an inhibitor of peptide synthesis, phenobarbital still inhibited the release of glycoproteins into the medium; however, the specific activity of liver glycoproteins was increased. The effects of phenobarbital on hepatic macromolecular secretion, independent of its effects on synthesis, were determined by prelabeling proteins in a liver slice system with either [14C]leucine of [14C]glucosamine. When phenobarbital was present, the secretion of these prelabeled proteins into the medium was impaired. 12 h after intraperitoneal injections of phenobarbital, glycoprotein secretion was inhibited from liver slices prepared from the pretreated rats. This inhibition of secretion occurred even though protein synthesis was stimulated and intracellular glycosylations unaffected. The results of this study indicate that phenobarbital impairs the secretion of glycoproteins by the liver.",
author = "Tuma, {Dean J.} and Jennett, {Richard B.} and Sorrell, {Michael F.}",
year = "1978",
month = "11",
day = "15",
doi = "10.1016/0304-4165(78)90218-0",
language = "English (US)",
volume = "544",
pages = "144--152",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0006-3002",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Impairment of glycoprotein secretion by phenobarbital in rat liver slices

AU - Tuma, Dean J.

AU - Jennett, Richard B.

AU - Sorrell, Michael F.

PY - 1978/11/15

Y1 - 1978/11/15

N2 - The effects of phenobarbital on protein and glycoprotein synthesis and secretion were studied in rat liver slices. Phenobarbital (2 mM) decreased [14C]-glucosamine and [14C]leucine incorporation into liver proteins and markedly inhibited their incorporation into medium (secretory) proteins. This inhibitory effect of phenobarbital was dose dependent and not reversible under the conditions of this study. In the presence of cycloheximide, an inhibitor of peptide synthesis, phenobarbital still inhibited the release of glycoproteins into the medium; however, the specific activity of liver glycoproteins was increased. The effects of phenobarbital on hepatic macromolecular secretion, independent of its effects on synthesis, were determined by prelabeling proteins in a liver slice system with either [14C]leucine of [14C]glucosamine. When phenobarbital was present, the secretion of these prelabeled proteins into the medium was impaired. 12 h after intraperitoneal injections of phenobarbital, glycoprotein secretion was inhibited from liver slices prepared from the pretreated rats. This inhibition of secretion occurred even though protein synthesis was stimulated and intracellular glycosylations unaffected. The results of this study indicate that phenobarbital impairs the secretion of glycoproteins by the liver.

AB - The effects of phenobarbital on protein and glycoprotein synthesis and secretion were studied in rat liver slices. Phenobarbital (2 mM) decreased [14C]-glucosamine and [14C]leucine incorporation into liver proteins and markedly inhibited their incorporation into medium (secretory) proteins. This inhibitory effect of phenobarbital was dose dependent and not reversible under the conditions of this study. In the presence of cycloheximide, an inhibitor of peptide synthesis, phenobarbital still inhibited the release of glycoproteins into the medium; however, the specific activity of liver glycoproteins was increased. The effects of phenobarbital on hepatic macromolecular secretion, independent of its effects on synthesis, were determined by prelabeling proteins in a liver slice system with either [14C]leucine of [14C]glucosamine. When phenobarbital was present, the secretion of these prelabeled proteins into the medium was impaired. 12 h after intraperitoneal injections of phenobarbital, glycoprotein secretion was inhibited from liver slices prepared from the pretreated rats. This inhibition of secretion occurred even though protein synthesis was stimulated and intracellular glycosylations unaffected. The results of this study indicate that phenobarbital impairs the secretion of glycoproteins by the liver.

UR - http://www.scopus.com/inward/record.url?scp=0018125905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018125905&partnerID=8YFLogxK

U2 - 10.1016/0304-4165(78)90218-0

DO - 10.1016/0304-4165(78)90218-0

M3 - Article

C2 - 718993

AN - SCOPUS:0018125905

VL - 544

SP - 144

EP - 152

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0006-3002

IS - 1

ER -