Impaired plasma membrane glycoprotein assembly in the liver following acute ethanol administration

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The in vivo effects of acute ethanol administration on hepatic plasma membrane assembly were studied in the rat. When [14C] fucose and [3H]N-acetylmannosamine, a sialic acid precursor, were injected following an acute dose of ethanol, minimal effects on fucose and a slight reduction of sialic acid incorporation into the total pool of hepatic membrane glycoproteins were observed. However, the assembly of labeled fucoproteins and sialoproteins into the plasma membrane was markedly inhibited in the ethanol-treated animals. These results indicate that ethanol administration impairs the late stages of membrane assembly which include the transport of glycoproteins from the Golgi complex to the plasma membrane and/or the insertion of glycoproteins into the membrane.

Original languageEnglish (US)
Pages (from-to)951-958
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume123
Issue number3
DOIs
StatePublished - Sep 28 1984

Fingerprint

Membrane Glycoproteins
Cell membranes
Liver
Ethanol
Cell Membrane
Fucose
N-Acetylneuraminic Acid
Glycoproteins
Sialoglycoproteins
Membranes
Golgi Apparatus
Rats
Animals

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{d8d7a769a749424b986ec5b9bd319e5d,
title = "Impaired plasma membrane glycoprotein assembly in the liver following acute ethanol administration",
abstract = "The in vivo effects of acute ethanol administration on hepatic plasma membrane assembly were studied in the rat. When [14C] fucose and [3H]N-acetylmannosamine, a sialic acid precursor, were injected following an acute dose of ethanol, minimal effects on fucose and a slight reduction of sialic acid incorporation into the total pool of hepatic membrane glycoproteins were observed. However, the assembly of labeled fucoproteins and sialoproteins into the plasma membrane was markedly inhibited in the ethanol-treated animals. These results indicate that ethanol administration impairs the late stages of membrane assembly which include the transport of glycoproteins from the Golgi complex to the plasma membrane and/or the insertion of glycoproteins into the membrane.",
author = "Mailliard, {Mark E.} and Sorrell, {Michael F.} and Volentine, {Gary D.} and Tuma, {Dean J.}",
year = "1984",
month = "9",
day = "28",
doi = "10.1016/S0006-291X(84)80226-0",
language = "English (US)",
volume = "123",
pages = "951--958",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Impaired plasma membrane glycoprotein assembly in the liver following acute ethanol administration

AU - Mailliard, Mark E.

AU - Sorrell, Michael F.

AU - Volentine, Gary D.

AU - Tuma, Dean J.

PY - 1984/9/28

Y1 - 1984/9/28

N2 - The in vivo effects of acute ethanol administration on hepatic plasma membrane assembly were studied in the rat. When [14C] fucose and [3H]N-acetylmannosamine, a sialic acid precursor, were injected following an acute dose of ethanol, minimal effects on fucose and a slight reduction of sialic acid incorporation into the total pool of hepatic membrane glycoproteins were observed. However, the assembly of labeled fucoproteins and sialoproteins into the plasma membrane was markedly inhibited in the ethanol-treated animals. These results indicate that ethanol administration impairs the late stages of membrane assembly which include the transport of glycoproteins from the Golgi complex to the plasma membrane and/or the insertion of glycoproteins into the membrane.

AB - The in vivo effects of acute ethanol administration on hepatic plasma membrane assembly were studied in the rat. When [14C] fucose and [3H]N-acetylmannosamine, a sialic acid precursor, were injected following an acute dose of ethanol, minimal effects on fucose and a slight reduction of sialic acid incorporation into the total pool of hepatic membrane glycoproteins were observed. However, the assembly of labeled fucoproteins and sialoproteins into the plasma membrane was markedly inhibited in the ethanol-treated animals. These results indicate that ethanol administration impairs the late stages of membrane assembly which include the transport of glycoproteins from the Golgi complex to the plasma membrane and/or the insertion of glycoproteins into the membrane.

UR - http://www.scopus.com/inward/record.url?scp=0021161648&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021161648&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(84)80226-0

DO - 10.1016/S0006-291X(84)80226-0

M3 - Article

C2 - 6487336

AN - SCOPUS:0021161648

VL - 123

SP - 951

EP - 958

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -