Impact of surface modifications of Acanthocheilonema viteae microfilariae on cell adhesion.

D. P. Singh, R. K. Chatterjee, V. M. Srivastava

Research output: Contribution to journalArticle

Abstract

Exposure of A. viteae microfilariae to various lectins reduced their capacity to react with the peritoneal exudate cells of the host, Mastomys natalensis. Sugars corresponding to these lectins with the exception of N-acetyl glucosamine, did not affect the adhesion per se. They however, protected the parasite against the adverse effect of lectins. Neuraminidase and chitinase also suppressed adhesion capacity of the microfilariae. Except sodium dodecylsulphate which enhanced cell attachment, other surfactants inhibited this reaction considerably. The results indicate that antibody dependent adhesion of the microfilariae with the macrophages involves surface moieties of the parasite, where N-acetylglucosamine acts as the principal sugar residue. Participation of -SH groups also is inferred from the observations that p-chloromercuribenzoate and dithiobis-(2-nitrobenzoic acid) inhibited cell attachment and dithiothreitol provided protection against these agents.

Original languageEnglish (US)
Pages (from-to)958-961
Number of pages4
JournalIndian journal of experimental biology
Volume29
Issue number10
StatePublished - Oct 1991

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Acanthocheilonema
Microfilariae
Cell adhesion
Lectins
Cell Adhesion
Surface treatment
Adhesion
Sugars
Parasites
Nitrobenzoates
Chloromercuribenzoates
Glucosamine
Murinae
Chitinases
Acetylglucosamine
Macrophages
Dithiothreitol
Neuraminidase
Exudates and Transudates
Antibodies

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Impact of surface modifications of Acanthocheilonema viteae microfilariae on cell adhesion. / Singh, D. P.; Chatterjee, R. K.; Srivastava, V. M.

In: Indian journal of experimental biology, Vol. 29, No. 10, 10.1991, p. 958-961.

Research output: Contribution to journalArticle

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