Impact of local heating and cooling on skeletal muscle transcriptional response related to myogenesis and proteolysis

Roksana B. Zak, B. M. Hassenstab, L. K. Zuehlke, M. W.S. Heesch, R. J. Shute, T. L. Laursen, D. T. LaSalle, D. R. Slivka

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Purpose: To determine the impact of local muscle heating and cooling on myogenic and proteolytic gene responses following resistance exercise. Methods: Recreationally trained males (n = 12), age 25.3 ± 1.5, % body fat 13.6 ± 1.92, completed four sets of 8–12 repetitions of unilateral leg press and leg extension while heating one leg, and cooling the other. Muscle biopsies were taken from the vastus lateralis of each leg pre and 4 h post exercise. Results: MyoD, FOXO1, and MuRF1 mRNA increased with exercise regardless of temperature (p < 0.05). Myostatin, MYF5, and atrogin-1 mRNA decreased with exercise regardless of temperature (p < 0.05). Myogenin, MRF4, and CASP3 mRNA were higher in the hot condition, compared to the cold (p < 0.05). PAX7 mRNA was lower in the hot compared to cold condition (p = 0.041). FOXO3 mRNA was higher in the cold compared to hot condition (p = 0.037). AKT1 and AKT2 were unaffected by either exercise or temperature. Femoral artery blood flow volume was higher in the hot (375.2 ± 41.2 ml min− 1), compared to the cold condition (263.5 ± 23.9 ml min− 1), p = 0.01. Tissue oxygen saturation was higher in the hot (71.7 ± 4.8%) than cold condition (55.3 ± 5.0%). Conclusion: These results suggest an impaired muscle growth response with local cold application compared to local heat application.

    Original languageEnglish (US)
    Pages (from-to)101-109
    Number of pages9
    JournalEuropean Journal of Applied Physiology
    Volume118
    Issue number1
    DOIs
    StatePublished - Jan 1 2018

    Fingerprint

    Muscle Development
    Heating
    Proteolysis
    Skeletal Muscle
    Leg
    Messenger RNA
    Muscles
    Temperature
    Myostatin
    Myogenin
    Quadriceps Muscle
    Femoral Artery
    Blood Volume
    Caspase 3
    Adipose Tissue
    Hot Temperature
    Oxygen
    Biopsy
    Growth
    Genes

    Keywords

    • Cold
    • Hot
    • Muscle breakdown
    • Muscle growth
    • Resistance exercise
    • Temperature

    ASJC Scopus subject areas

    • Orthopedics and Sports Medicine
    • Public Health, Environmental and Occupational Health
    • Physiology (medical)

    Cite this

    Impact of local heating and cooling on skeletal muscle transcriptional response related to myogenesis and proteolysis. / Zak, Roksana B.; Hassenstab, B. M.; Zuehlke, L. K.; Heesch, M. W.S.; Shute, R. J.; Laursen, T. L.; LaSalle, D. T.; Slivka, D. R.

    In: European Journal of Applied Physiology, Vol. 118, No. 1, 01.01.2018, p. 101-109.

    Research output: Contribution to journalArticle

    Zak, Roksana B. ; Hassenstab, B. M. ; Zuehlke, L. K. ; Heesch, M. W.S. ; Shute, R. J. ; Laursen, T. L. ; LaSalle, D. T. ; Slivka, D. R. / Impact of local heating and cooling on skeletal muscle transcriptional response related to myogenesis and proteolysis. In: European Journal of Applied Physiology. 2018 ; Vol. 118, No. 1. pp. 101-109.
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    abstract = "Purpose: To determine the impact of local muscle heating and cooling on myogenic and proteolytic gene responses following resistance exercise. Methods: Recreationally trained males (n = 12), age 25.3 ± 1.5, {\%} body fat 13.6 ± 1.92, completed four sets of 8–12 repetitions of unilateral leg press and leg extension while heating one leg, and cooling the other. Muscle biopsies were taken from the vastus lateralis of each leg pre and 4 h post exercise. Results: MyoD, FOXO1, and MuRF1 mRNA increased with exercise regardless of temperature (p < 0.05). Myostatin, MYF5, and atrogin-1 mRNA decreased with exercise regardless of temperature (p < 0.05). Myogenin, MRF4, and CASP3 mRNA were higher in the hot condition, compared to the cold (p < 0.05). PAX7 mRNA was lower in the hot compared to cold condition (p = 0.041). FOXO3 mRNA was higher in the cold compared to hot condition (p = 0.037). AKT1 and AKT2 were unaffected by either exercise or temperature. Femoral artery blood flow volume was higher in the hot (375.2 ± 41.2 ml min− 1), compared to the cold condition (263.5 ± 23.9 ml min− 1), p = 0.01. Tissue oxygen saturation was higher in the hot (71.7 ± 4.8{\%}) than cold condition (55.3 ± 5.0{\%}). Conclusion: These results suggest an impaired muscle growth response with local cold application compared to local heat application.",
    keywords = "Cold, Hot, Muscle breakdown, Muscle growth, Resistance exercise, Temperature",
    author = "Zak, {Roksana B.} and Hassenstab, {B. M.} and Zuehlke, {L. K.} and Heesch, {M. W.S.} and Shute, {R. J.} and Laursen, {T. L.} and LaSalle, {D. T.} and Slivka, {D. R.}",
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    T1 - Impact of local heating and cooling on skeletal muscle transcriptional response related to myogenesis and proteolysis

    AU - Zak, Roksana B.

    AU - Hassenstab, B. M.

    AU - Zuehlke, L. K.

    AU - Heesch, M. W.S.

    AU - Shute, R. J.

    AU - Laursen, T. L.

    AU - LaSalle, D. T.

    AU - Slivka, D. R.

    PY - 2018/1/1

    Y1 - 2018/1/1

    N2 - Purpose: To determine the impact of local muscle heating and cooling on myogenic and proteolytic gene responses following resistance exercise. Methods: Recreationally trained males (n = 12), age 25.3 ± 1.5, % body fat 13.6 ± 1.92, completed four sets of 8–12 repetitions of unilateral leg press and leg extension while heating one leg, and cooling the other. Muscle biopsies were taken from the vastus lateralis of each leg pre and 4 h post exercise. Results: MyoD, FOXO1, and MuRF1 mRNA increased with exercise regardless of temperature (p < 0.05). Myostatin, MYF5, and atrogin-1 mRNA decreased with exercise regardless of temperature (p < 0.05). Myogenin, MRF4, and CASP3 mRNA were higher in the hot condition, compared to the cold (p < 0.05). PAX7 mRNA was lower in the hot compared to cold condition (p = 0.041). FOXO3 mRNA was higher in the cold compared to hot condition (p = 0.037). AKT1 and AKT2 were unaffected by either exercise or temperature. Femoral artery blood flow volume was higher in the hot (375.2 ± 41.2 ml min− 1), compared to the cold condition (263.5 ± 23.9 ml min− 1), p = 0.01. Tissue oxygen saturation was higher in the hot (71.7 ± 4.8%) than cold condition (55.3 ± 5.0%). Conclusion: These results suggest an impaired muscle growth response with local cold application compared to local heat application.

    AB - Purpose: To determine the impact of local muscle heating and cooling on myogenic and proteolytic gene responses following resistance exercise. Methods: Recreationally trained males (n = 12), age 25.3 ± 1.5, % body fat 13.6 ± 1.92, completed four sets of 8–12 repetitions of unilateral leg press and leg extension while heating one leg, and cooling the other. Muscle biopsies were taken from the vastus lateralis of each leg pre and 4 h post exercise. Results: MyoD, FOXO1, and MuRF1 mRNA increased with exercise regardless of temperature (p < 0.05). Myostatin, MYF5, and atrogin-1 mRNA decreased with exercise regardless of temperature (p < 0.05). Myogenin, MRF4, and CASP3 mRNA were higher in the hot condition, compared to the cold (p < 0.05). PAX7 mRNA was lower in the hot compared to cold condition (p = 0.041). FOXO3 mRNA was higher in the cold compared to hot condition (p = 0.037). AKT1 and AKT2 were unaffected by either exercise or temperature. Femoral artery blood flow volume was higher in the hot (375.2 ± 41.2 ml min− 1), compared to the cold condition (263.5 ± 23.9 ml min− 1), p = 0.01. Tissue oxygen saturation was higher in the hot (71.7 ± 4.8%) than cold condition (55.3 ± 5.0%). Conclusion: These results suggest an impaired muscle growth response with local cold application compared to local heat application.

    KW - Cold

    KW - Hot

    KW - Muscle breakdown

    KW - Muscle growth

    KW - Resistance exercise

    KW - Temperature

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    U2 - 10.1007/s00421-017-3749-z

    DO - 10.1007/s00421-017-3749-z

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