Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice

Stephen B. Smith, Zhaobin Xu, Tatiana Novitskaya, Bo Zhang, Elena Chepurko, Xin An Pu, Debra G. Wheeler, Mark Ziolo, Richard J. Gumina

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aims Prior work suggests that ischemic preconditioning increases the level of CD39 in the heart and contributes to cardiac protection. Therefore, we examined if targeted cardiac expression of CD39 protects against myocardial injury. Main methods Mice with cardiac-specific expression of human CD39 (αMHC/hCD39-Tg) were generated, characterized and subjected to left coronary artery ischemia-reperfusion injury and infarct size at 24 h following injury quantified. Key findings αMHC/hCD39-Tg mice have increased in cardiac ATPase and ADPase activity compared to WT littermates. The increased activity in αMHC/hCD39-mice was inhibited by the CD39 antagonist sodium polyoxotungstate (POM-1). Measurement of basal cardiac function by echocardiography revealed that αMHC/hCD39-Tg mice have a lower resting heart rate and increased stroke volume. In response to myocardial ischemia, systolic and diastolic function was better preserved in αMHC/hCD39-Tg compared to WT mice. Comparison of Tau also revealed preserved cardiac relaxation during ischemia in αMHC/hCD39-Tg hearts. Assessment of myocardial infarct size in response to 60 min of ischemia and 24 h of reperfusion demonstrated a significant reduction in infarct size in αMHC/hCD39-Tg hearts. Analysis of isolated cardiomyocytes revealed no basal difference in calcium transients between WT and αMHC/hCD39-Tg cardiomyocytes. However, in response to isoproterenol stimulation, there was a trend toward lower calcium transients in αMHC/hCD39 cardiomyocytes suggesting less calcium accumulation in response to metabolic stress. Significance Cardiac-specific expression of CD39 reduces myocardial dysfunction and infarct size following ischemia-reperfusion injury. Increasing nucleotidase expression in the heart may be a novel approach to protect the heart from ischemic injury.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalLife Sciences
Volume179
DOIs
StatePublished - Jun 15 2017

Fingerprint

Myocardial Infarction
Cardiac Myocytes
nucleotidase
Calcium
Reperfusion Injury
Ischemia
Apyrase
Heart Injuries
Ischemic Preconditioning
Physiological Stress
Wounds and Injuries
Echocardiography
Isoproterenol
Stroke Volume
Reperfusion
Myocardial Ischemia
Adenosine Triphosphatases
Coronary Vessels
Heart Rate
Sodium

Keywords

  • CD39
  • Infarct
  • Ischemia-reperfusion

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Smith, S. B., Xu, Z., Novitskaya, T., Zhang, B., Chepurko, E., Pu, X. A., ... Gumina, R. J. (2017). Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice. Life Sciences, 179, 54-59. https://doi.org/10.1016/j.lfs.2016.10.016

Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice. / Smith, Stephen B.; Xu, Zhaobin; Novitskaya, Tatiana; Zhang, Bo; Chepurko, Elena; Pu, Xin An; Wheeler, Debra G.; Ziolo, Mark; Gumina, Richard J.

In: Life Sciences, Vol. 179, 15.06.2017, p. 54-59.

Research output: Contribution to journalArticle

Smith, SB, Xu, Z, Novitskaya, T, Zhang, B, Chepurko, E, Pu, XA, Wheeler, DG, Ziolo, M & Gumina, RJ 2017, 'Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice', Life Sciences, vol. 179, pp. 54-59. https://doi.org/10.1016/j.lfs.2016.10.016
Smith SB, Xu Z, Novitskaya T, Zhang B, Chepurko E, Pu XA et al. Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice. Life Sciences. 2017 Jun 15;179:54-59. https://doi.org/10.1016/j.lfs.2016.10.016
Smith, Stephen B. ; Xu, Zhaobin ; Novitskaya, Tatiana ; Zhang, Bo ; Chepurko, Elena ; Pu, Xin An ; Wheeler, Debra G. ; Ziolo, Mark ; Gumina, Richard J. / Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice. In: Life Sciences. 2017 ; Vol. 179. pp. 54-59.
@article{0cc63be4e95548eeb9f5d68424ab73ed,
title = "Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice",
abstract = "Aims Prior work suggests that ischemic preconditioning increases the level of CD39 in the heart and contributes to cardiac protection. Therefore, we examined if targeted cardiac expression of CD39 protects against myocardial injury. Main methods Mice with cardiac-specific expression of human CD39 (αMHC/hCD39-Tg) were generated, characterized and subjected to left coronary artery ischemia-reperfusion injury and infarct size at 24 h following injury quantified. Key findings αMHC/hCD39-Tg mice have increased in cardiac ATPase and ADPase activity compared to WT littermates. The increased activity in αMHC/hCD39-mice was inhibited by the CD39 antagonist sodium polyoxotungstate (POM-1). Measurement of basal cardiac function by echocardiography revealed that αMHC/hCD39-Tg mice have a lower resting heart rate and increased stroke volume. In response to myocardial ischemia, systolic and diastolic function was better preserved in αMHC/hCD39-Tg compared to WT mice. Comparison of Tau also revealed preserved cardiac relaxation during ischemia in αMHC/hCD39-Tg hearts. Assessment of myocardial infarct size in response to 60 min of ischemia and 24 h of reperfusion demonstrated a significant reduction in infarct size in αMHC/hCD39-Tg hearts. Analysis of isolated cardiomyocytes revealed no basal difference in calcium transients between WT and αMHC/hCD39-Tg cardiomyocytes. However, in response to isoproterenol stimulation, there was a trend toward lower calcium transients in αMHC/hCD39 cardiomyocytes suggesting less calcium accumulation in response to metabolic stress. Significance Cardiac-specific expression of CD39 reduces myocardial dysfunction and infarct size following ischemia-reperfusion injury. Increasing nucleotidase expression in the heart may be a novel approach to protect the heart from ischemic injury.",
keywords = "CD39, Infarct, Ischemia-reperfusion",
author = "Smith, {Stephen B.} and Zhaobin Xu and Tatiana Novitskaya and Bo Zhang and Elena Chepurko and Pu, {Xin An} and Wheeler, {Debra G.} and Mark Ziolo and Gumina, {Richard J.}",
year = "2017",
month = "6",
day = "15",
doi = "10.1016/j.lfs.2016.10.016",
language = "English (US)",
volume = "179",
pages = "54--59",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Impact of cardiac-specific expression of CD39 on myocardial infarct size in mice

AU - Smith, Stephen B.

AU - Xu, Zhaobin

AU - Novitskaya, Tatiana

AU - Zhang, Bo

AU - Chepurko, Elena

AU - Pu, Xin An

AU - Wheeler, Debra G.

AU - Ziolo, Mark

AU - Gumina, Richard J.

PY - 2017/6/15

Y1 - 2017/6/15

N2 - Aims Prior work suggests that ischemic preconditioning increases the level of CD39 in the heart and contributes to cardiac protection. Therefore, we examined if targeted cardiac expression of CD39 protects against myocardial injury. Main methods Mice with cardiac-specific expression of human CD39 (αMHC/hCD39-Tg) were generated, characterized and subjected to left coronary artery ischemia-reperfusion injury and infarct size at 24 h following injury quantified. Key findings αMHC/hCD39-Tg mice have increased in cardiac ATPase and ADPase activity compared to WT littermates. The increased activity in αMHC/hCD39-mice was inhibited by the CD39 antagonist sodium polyoxotungstate (POM-1). Measurement of basal cardiac function by echocardiography revealed that αMHC/hCD39-Tg mice have a lower resting heart rate and increased stroke volume. In response to myocardial ischemia, systolic and diastolic function was better preserved in αMHC/hCD39-Tg compared to WT mice. Comparison of Tau also revealed preserved cardiac relaxation during ischemia in αMHC/hCD39-Tg hearts. Assessment of myocardial infarct size in response to 60 min of ischemia and 24 h of reperfusion demonstrated a significant reduction in infarct size in αMHC/hCD39-Tg hearts. Analysis of isolated cardiomyocytes revealed no basal difference in calcium transients between WT and αMHC/hCD39-Tg cardiomyocytes. However, in response to isoproterenol stimulation, there was a trend toward lower calcium transients in αMHC/hCD39 cardiomyocytes suggesting less calcium accumulation in response to metabolic stress. Significance Cardiac-specific expression of CD39 reduces myocardial dysfunction and infarct size following ischemia-reperfusion injury. Increasing nucleotidase expression in the heart may be a novel approach to protect the heart from ischemic injury.

AB - Aims Prior work suggests that ischemic preconditioning increases the level of CD39 in the heart and contributes to cardiac protection. Therefore, we examined if targeted cardiac expression of CD39 protects against myocardial injury. Main methods Mice with cardiac-specific expression of human CD39 (αMHC/hCD39-Tg) were generated, characterized and subjected to left coronary artery ischemia-reperfusion injury and infarct size at 24 h following injury quantified. Key findings αMHC/hCD39-Tg mice have increased in cardiac ATPase and ADPase activity compared to WT littermates. The increased activity in αMHC/hCD39-mice was inhibited by the CD39 antagonist sodium polyoxotungstate (POM-1). Measurement of basal cardiac function by echocardiography revealed that αMHC/hCD39-Tg mice have a lower resting heart rate and increased stroke volume. In response to myocardial ischemia, systolic and diastolic function was better preserved in αMHC/hCD39-Tg compared to WT mice. Comparison of Tau also revealed preserved cardiac relaxation during ischemia in αMHC/hCD39-Tg hearts. Assessment of myocardial infarct size in response to 60 min of ischemia and 24 h of reperfusion demonstrated a significant reduction in infarct size in αMHC/hCD39-Tg hearts. Analysis of isolated cardiomyocytes revealed no basal difference in calcium transients between WT and αMHC/hCD39-Tg cardiomyocytes. However, in response to isoproterenol stimulation, there was a trend toward lower calcium transients in αMHC/hCD39 cardiomyocytes suggesting less calcium accumulation in response to metabolic stress. Significance Cardiac-specific expression of CD39 reduces myocardial dysfunction and infarct size following ischemia-reperfusion injury. Increasing nucleotidase expression in the heart may be a novel approach to protect the heart from ischemic injury.

KW - CD39

KW - Infarct

KW - Ischemia-reperfusion

UR - http://www.scopus.com/inward/record.url?scp=85018397560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018397560&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2016.10.016

DO - 10.1016/j.lfs.2016.10.016

M3 - Article

C2 - 27756600

AN - SCOPUS:85018397560

VL - 179

SP - 54

EP - 59

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

ER -