Impact of beta 2-microglobulin on tapasin expression and covalent association

Laura C. Simone, Brittney L. Smith, Joyce C Solheim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cellular immunity is dependent on major histocompatibility complex (MHC) class I molecules enabling cytotoxic T cell recognition of malignant and infected cells. Loading of antigenic peptides onto MHC class I is assisted by a peptide-loading protein complex including tapasin. We found that tapasin expression is enhanced by beta 2-microglobulin via both transcriptional and post-transcriptional mechanisms. In addition, using conditions which preserve the tapasin-ERp57 disulfide-bonded conjugate, we demonstrated that beta 2-microglobulin increases tapasin-containing protein complexes, and reduces the level of MHC class I/ERp57 complexes lacking tapasin. Overall, our results provide a new perspective on the regulation of tapasin expression and association.

Original languageEnglish (US)
Pages (from-to)66-69
Number of pages4
JournalCellular Immunology
Volume279
Issue number1
DOIs
StatePublished - Sep 1 2012

Fingerprint

beta 2-Microglobulin
Major Histocompatibility Complex
Peptides
Cellular Immunity
Disulfides
tapasin
Proteins
T-Lymphocytes

Keywords

  • Antigen presentation
  • Beta 2-microglobulin
  • D
  • ERp57
  • Major histocompatibility complex class I
  • Peptide-loading complex
  • Tapasin

ASJC Scopus subject areas

  • Immunology

Cite this

Impact of beta 2-microglobulin on tapasin expression and covalent association. / Simone, Laura C.; Smith, Brittney L.; Solheim, Joyce C.

In: Cellular Immunology, Vol. 279, No. 1, 01.09.2012, p. 66-69.

Research output: Contribution to journalArticle

Simone, Laura C. ; Smith, Brittney L. ; Solheim, Joyce C. / Impact of beta 2-microglobulin on tapasin expression and covalent association. In: Cellular Immunology. 2012 ; Vol. 279, No. 1. pp. 66-69.
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