Immunoselection of tumor variants resistant to antibody-mediated cytotoxicity - Their immunologic and metastatic characterization

Jean R. Starkey, William C. Davis, James E. Talmadge

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The immunological characteristics of two series of metastatic variants of restricted genetic origin were related to their lung-colony-forming potential. A series of metastatic variants was isolated from a tumor-cell population in which heterogeneity appeared following short-term in vivo passage, while a second series of variants were immunoselected in vitro for resistance to antibody-complement-mediated cell lysis. In the case of the first series, the sensitivity of the individual variants to cell-mediated and antibody-complement-mediated cytotoxicity appeared to be partly determined by the number and rate of loss of antibody-binding sites on the cell surface. These characteristics also correlated with the efficiency of experimental metastasis in the animal. We compared the results reported herein with our previous studies of nonimmune parameters for this series of metastatic variants, and we conclude that immunological differences can be important to the efficiency of lung-colony formation. However, in the case of the second series of variants, despite selection in vitro for resistance to antibody-complement-mediated cell lysis, the behavior of these variants in the lung colony assay could not be predicted by the immunologic parameters examined.

Original languageEnglish (US)
Pages (from-to)124-131
Number of pages8
JournalCancer Immunology Immunotherapy
Volume14
Issue number2
DOIs
StatePublished - Dec 1 1982

Fingerprint

Immunologic Cytotoxicity
Antibodies
Neoplasms
Lung
Antibody Binding Sites
Neoplasm Metastasis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

Cite this

Immunoselection of tumor variants resistant to antibody-mediated cytotoxicity - Their immunologic and metastatic characterization. / Starkey, Jean R.; Davis, William C.; Talmadge, James E.

In: Cancer Immunology Immunotherapy, Vol. 14, No. 2, 01.12.1982, p. 124-131.

Research output: Contribution to journalArticle

@article{8e8135c0eeb54439a34c491730ba5c86,
title = "Immunoselection of tumor variants resistant to antibody-mediated cytotoxicity - Their immunologic and metastatic characterization",
abstract = "The immunological characteristics of two series of metastatic variants of restricted genetic origin were related to their lung-colony-forming potential. A series of metastatic variants was isolated from a tumor-cell population in which heterogeneity appeared following short-term in vivo passage, while a second series of variants were immunoselected in vitro for resistance to antibody-complement-mediated cell lysis. In the case of the first series, the sensitivity of the individual variants to cell-mediated and antibody-complement-mediated cytotoxicity appeared to be partly determined by the number and rate of loss of antibody-binding sites on the cell surface. These characteristics also correlated with the efficiency of experimental metastasis in the animal. We compared the results reported herein with our previous studies of nonimmune parameters for this series of metastatic variants, and we conclude that immunological differences can be important to the efficiency of lung-colony formation. However, in the case of the second series of variants, despite selection in vitro for resistance to antibody-complement-mediated cell lysis, the behavior of these variants in the lung colony assay could not be predicted by the immunologic parameters examined.",
author = "Starkey, {Jean R.} and Davis, {William C.} and Talmadge, {James E.}",
year = "1982",
month = "12",
day = "1",
doi = "10.1007/BF00200180",
language = "English (US)",
volume = "14",
pages = "124--131",
journal = "Cancer Immunology and Immunotherapy",
issn = "0340-7004",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "2",

}

TY - JOUR

T1 - Immunoselection of tumor variants resistant to antibody-mediated cytotoxicity - Their immunologic and metastatic characterization

AU - Starkey, Jean R.

AU - Davis, William C.

AU - Talmadge, James E.

PY - 1982/12/1

Y1 - 1982/12/1

N2 - The immunological characteristics of two series of metastatic variants of restricted genetic origin were related to their lung-colony-forming potential. A series of metastatic variants was isolated from a tumor-cell population in which heterogeneity appeared following short-term in vivo passage, while a second series of variants were immunoselected in vitro for resistance to antibody-complement-mediated cell lysis. In the case of the first series, the sensitivity of the individual variants to cell-mediated and antibody-complement-mediated cytotoxicity appeared to be partly determined by the number and rate of loss of antibody-binding sites on the cell surface. These characteristics also correlated with the efficiency of experimental metastasis in the animal. We compared the results reported herein with our previous studies of nonimmune parameters for this series of metastatic variants, and we conclude that immunological differences can be important to the efficiency of lung-colony formation. However, in the case of the second series of variants, despite selection in vitro for resistance to antibody-complement-mediated cell lysis, the behavior of these variants in the lung colony assay could not be predicted by the immunologic parameters examined.

AB - The immunological characteristics of two series of metastatic variants of restricted genetic origin were related to their lung-colony-forming potential. A series of metastatic variants was isolated from a tumor-cell population in which heterogeneity appeared following short-term in vivo passage, while a second series of variants were immunoselected in vitro for resistance to antibody-complement-mediated cell lysis. In the case of the first series, the sensitivity of the individual variants to cell-mediated and antibody-complement-mediated cytotoxicity appeared to be partly determined by the number and rate of loss of antibody-binding sites on the cell surface. These characteristics also correlated with the efficiency of experimental metastasis in the animal. We compared the results reported herein with our previous studies of nonimmune parameters for this series of metastatic variants, and we conclude that immunological differences can be important to the efficiency of lung-colony formation. However, in the case of the second series of variants, despite selection in vitro for resistance to antibody-complement-mediated cell lysis, the behavior of these variants in the lung colony assay could not be predicted by the immunologic parameters examined.

UR - http://www.scopus.com/inward/record.url?scp=0020372018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020372018&partnerID=8YFLogxK

U2 - 10.1007/BF00200180

DO - 10.1007/BF00200180

M3 - Article

C2 - 6965227

AN - SCOPUS:0020372018

VL - 14

SP - 124

EP - 131

JO - Cancer Immunology and Immunotherapy

JF - Cancer Immunology and Immunotherapy

SN - 0340-7004

IS - 2

ER -