Immunophenotypes and cytogenetic alterations in 219 cases of diffuse large B-cell lymphoma

Bingxin Guan, Yi Pan, Aiju Fang, Yan Liang, Yingying Huo, Baocun Sun, Huaqing Wang, Kai Fu, Bin Meng

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the immunophenotypes, subtypes based on Hans algorithm, and alterations of Bcl-2 and Bcl-6 gene in diffuse large B-cell lymphoma (DLBCL) from Chinese patients. Methods: The expression of CD10, BCL-6, MUM1, BCL-2, Ki67 and other markers were detected by immunohistochemistry in tissue microarrays of 219 DLBCL cases. Hans algorithm was applied to classify DLBCL into GCB and non-GCB subtypes. Interphase fluorescence in situ hybridization (FISH) was used to detect the incidence of Bcl-2 and Bcl-6 gene alterations. Other seven studies published in the last two years on DLBCL from China were collected and analyzed. Results: (1) This study: Circled digit one of 219 DLBCL cases, non-GCB subtype (165 cases, 75.3 %) was significantly more common than GCB subtype (54 cases, 24.7 %) (P < 0.001). Circled digit two Bcl-2 gene alteration was detected in 49 cases (25.8 %), including 5 cases with the t (14;18) (2.6 %) which all existed in GCB subtype, and 44 cases of Bcl-2 amplification (23.2 %) which existed in GCB subtype in 4 cases (8.5 %) and in non-GCB subtype in 40 cases (28.0 %) (P = 0.013). Circled digit three Bcl-6 gene rearrangement was detected in 42 cases (22.1 %), which was in GCB in 7 cases (14.9 %) and was in non-GCB in 35 cases (24.5 %) (P = 0.189). Circled digit four a significant negative correlation between Bcl-2 gene amplification and Bcl-6 gene rearrangement was seen (r = -0.179 6, P = 0.016 8). Amongst 5 cases having the t (14;18) translocation, 2 cases accompanied by Bcl-6 gene rearrangement. Forty-nine cases with Bcl-2 gene alterations including translocation and amplification were all but one expressing BCL-2 protein, and there was no significant association between Bcl-6 gene rearrangement and BCL-6 protein expression (P = 0.726). Circled digit five there was no significant difference between nodal and extranodal DLBCLs in immunophenotypes, subtypes based on Hans algorithm, and Bcl-2 and Bcl-6 gene alterations (P = 0.462, P = 0.426 and P = 0.167). (2) Comprehensive analysis of eight studies: of the total 1, 259 cases, non-GCB subtype (879 cases, 69.8 %) was significantly more common than GCB subtype (380 cases, 30.2 %) (P < 0.001). Conclusion: There are some distinctive features on immunophenotypes and cytogenetic alterations in DLBCL of Chinese patients, which need elucidation by more studies.

Original languageEnglish (US)
Pages (from-to)829-833
Number of pages5
JournalChinese Journal of Clinical Oncology
Volume39
Issue number12
DOIs
StatePublished - Jun 30 2012

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bcl-2 Genes
Lymphoma, Large B-Cell, Diffuse
Cytogenetics
Gene Rearrangement
Gene Amplification
Interphase
Fluorescence In Situ Hybridization
China
Proteins
Immunohistochemistry
Incidence

Keywords

  • Bcl-2 gene
  • Bcl-6 gene
  • Diffuse large B-cell lymphoma
  • Immunophenotype

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Immunophenotypes and cytogenetic alterations in 219 cases of diffuse large B-cell lymphoma. / Guan, Bingxin; Pan, Yi; Fang, Aiju; Liang, Yan; Huo, Yingying; Sun, Baocun; Wang, Huaqing; Fu, Kai; Meng, Bin.

In: Chinese Journal of Clinical Oncology, Vol. 39, No. 12, 30.06.2012, p. 829-833.

Research output: Contribution to journalArticle

Guan, Bingxin ; Pan, Yi ; Fang, Aiju ; Liang, Yan ; Huo, Yingying ; Sun, Baocun ; Wang, Huaqing ; Fu, Kai ; Meng, Bin. / Immunophenotypes and cytogenetic alterations in 219 cases of diffuse large B-cell lymphoma. In: Chinese Journal of Clinical Oncology. 2012 ; Vol. 39, No. 12. pp. 829-833.
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title = "Immunophenotypes and cytogenetic alterations in 219 cases of diffuse large B-cell lymphoma",
abstract = "Objective: To investigate the immunophenotypes, subtypes based on Hans algorithm, and alterations of Bcl-2 and Bcl-6 gene in diffuse large B-cell lymphoma (DLBCL) from Chinese patients. Methods: The expression of CD10, BCL-6, MUM1, BCL-2, Ki67 and other markers were detected by immunohistochemistry in tissue microarrays of 219 DLBCL cases. Hans algorithm was applied to classify DLBCL into GCB and non-GCB subtypes. Interphase fluorescence in situ hybridization (FISH) was used to detect the incidence of Bcl-2 and Bcl-6 gene alterations. Other seven studies published in the last two years on DLBCL from China were collected and analyzed. Results: (1) This study: Circled digit one of 219 DLBCL cases, non-GCB subtype (165 cases, 75.3 {\%}) was significantly more common than GCB subtype (54 cases, 24.7 {\%}) (P < 0.001). Circled digit two Bcl-2 gene alteration was detected in 49 cases (25.8 {\%}), including 5 cases with the t (14;18) (2.6 {\%}) which all existed in GCB subtype, and 44 cases of Bcl-2 amplification (23.2 {\%}) which existed in GCB subtype in 4 cases (8.5 {\%}) and in non-GCB subtype in 40 cases (28.0 {\%}) (P = 0.013). Circled digit three Bcl-6 gene rearrangement was detected in 42 cases (22.1 {\%}), which was in GCB in 7 cases (14.9 {\%}) and was in non-GCB in 35 cases (24.5 {\%}) (P = 0.189). Circled digit four a significant negative correlation between Bcl-2 gene amplification and Bcl-6 gene rearrangement was seen (r = -0.179 6, P = 0.016 8). Amongst 5 cases having the t (14;18) translocation, 2 cases accompanied by Bcl-6 gene rearrangement. Forty-nine cases with Bcl-2 gene alterations including translocation and amplification were all but one expressing BCL-2 protein, and there was no significant association between Bcl-6 gene rearrangement and BCL-6 protein expression (P = 0.726). Circled digit five there was no significant difference between nodal and extranodal DLBCLs in immunophenotypes, subtypes based on Hans algorithm, and Bcl-2 and Bcl-6 gene alterations (P = 0.462, P = 0.426 and P = 0.167). (2) Comprehensive analysis of eight studies: of the total 1, 259 cases, non-GCB subtype (879 cases, 69.8 {\%}) was significantly more common than GCB subtype (380 cases, 30.2 {\%}) (P < 0.001). Conclusion: There are some distinctive features on immunophenotypes and cytogenetic alterations in DLBCL of Chinese patients, which need elucidation by more studies.",
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author = "Bingxin Guan and Yi Pan and Aiju Fang and Yan Liang and Yingying Huo and Baocun Sun and Huaqing Wang and Kai Fu and Bin Meng",
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TY - JOUR

T1 - Immunophenotypes and cytogenetic alterations in 219 cases of diffuse large B-cell lymphoma

AU - Guan, Bingxin

AU - Pan, Yi

AU - Fang, Aiju

AU - Liang, Yan

AU - Huo, Yingying

AU - Sun, Baocun

AU - Wang, Huaqing

AU - Fu, Kai

AU - Meng, Bin

PY - 2012/6/30

Y1 - 2012/6/30

N2 - Objective: To investigate the immunophenotypes, subtypes based on Hans algorithm, and alterations of Bcl-2 and Bcl-6 gene in diffuse large B-cell lymphoma (DLBCL) from Chinese patients. Methods: The expression of CD10, BCL-6, MUM1, BCL-2, Ki67 and other markers were detected by immunohistochemistry in tissue microarrays of 219 DLBCL cases. Hans algorithm was applied to classify DLBCL into GCB and non-GCB subtypes. Interphase fluorescence in situ hybridization (FISH) was used to detect the incidence of Bcl-2 and Bcl-6 gene alterations. Other seven studies published in the last two years on DLBCL from China were collected and analyzed. Results: (1) This study: Circled digit one of 219 DLBCL cases, non-GCB subtype (165 cases, 75.3 %) was significantly more common than GCB subtype (54 cases, 24.7 %) (P < 0.001). Circled digit two Bcl-2 gene alteration was detected in 49 cases (25.8 %), including 5 cases with the t (14;18) (2.6 %) which all existed in GCB subtype, and 44 cases of Bcl-2 amplification (23.2 %) which existed in GCB subtype in 4 cases (8.5 %) and in non-GCB subtype in 40 cases (28.0 %) (P = 0.013). Circled digit three Bcl-6 gene rearrangement was detected in 42 cases (22.1 %), which was in GCB in 7 cases (14.9 %) and was in non-GCB in 35 cases (24.5 %) (P = 0.189). Circled digit four a significant negative correlation between Bcl-2 gene amplification and Bcl-6 gene rearrangement was seen (r = -0.179 6, P = 0.016 8). Amongst 5 cases having the t (14;18) translocation, 2 cases accompanied by Bcl-6 gene rearrangement. Forty-nine cases with Bcl-2 gene alterations including translocation and amplification were all but one expressing BCL-2 protein, and there was no significant association between Bcl-6 gene rearrangement and BCL-6 protein expression (P = 0.726). Circled digit five there was no significant difference between nodal and extranodal DLBCLs in immunophenotypes, subtypes based on Hans algorithm, and Bcl-2 and Bcl-6 gene alterations (P = 0.462, P = 0.426 and P = 0.167). (2) Comprehensive analysis of eight studies: of the total 1, 259 cases, non-GCB subtype (879 cases, 69.8 %) was significantly more common than GCB subtype (380 cases, 30.2 %) (P < 0.001). Conclusion: There are some distinctive features on immunophenotypes and cytogenetic alterations in DLBCL of Chinese patients, which need elucidation by more studies.

AB - Objective: To investigate the immunophenotypes, subtypes based on Hans algorithm, and alterations of Bcl-2 and Bcl-6 gene in diffuse large B-cell lymphoma (DLBCL) from Chinese patients. Methods: The expression of CD10, BCL-6, MUM1, BCL-2, Ki67 and other markers were detected by immunohistochemistry in tissue microarrays of 219 DLBCL cases. Hans algorithm was applied to classify DLBCL into GCB and non-GCB subtypes. Interphase fluorescence in situ hybridization (FISH) was used to detect the incidence of Bcl-2 and Bcl-6 gene alterations. Other seven studies published in the last two years on DLBCL from China were collected and analyzed. Results: (1) This study: Circled digit one of 219 DLBCL cases, non-GCB subtype (165 cases, 75.3 %) was significantly more common than GCB subtype (54 cases, 24.7 %) (P < 0.001). Circled digit two Bcl-2 gene alteration was detected in 49 cases (25.8 %), including 5 cases with the t (14;18) (2.6 %) which all existed in GCB subtype, and 44 cases of Bcl-2 amplification (23.2 %) which existed in GCB subtype in 4 cases (8.5 %) and in non-GCB subtype in 40 cases (28.0 %) (P = 0.013). Circled digit three Bcl-6 gene rearrangement was detected in 42 cases (22.1 %), which was in GCB in 7 cases (14.9 %) and was in non-GCB in 35 cases (24.5 %) (P = 0.189). Circled digit four a significant negative correlation between Bcl-2 gene amplification and Bcl-6 gene rearrangement was seen (r = -0.179 6, P = 0.016 8). Amongst 5 cases having the t (14;18) translocation, 2 cases accompanied by Bcl-6 gene rearrangement. Forty-nine cases with Bcl-2 gene alterations including translocation and amplification were all but one expressing BCL-2 protein, and there was no significant association between Bcl-6 gene rearrangement and BCL-6 protein expression (P = 0.726). Circled digit five there was no significant difference between nodal and extranodal DLBCLs in immunophenotypes, subtypes based on Hans algorithm, and Bcl-2 and Bcl-6 gene alterations (P = 0.462, P = 0.426 and P = 0.167). (2) Comprehensive analysis of eight studies: of the total 1, 259 cases, non-GCB subtype (879 cases, 69.8 %) was significantly more common than GCB subtype (380 cases, 30.2 %) (P < 0.001). Conclusion: There are some distinctive features on immunophenotypes and cytogenetic alterations in DLBCL of Chinese patients, which need elucidation by more studies.

KW - Bcl-2 gene

KW - Bcl-6 gene

KW - Diffuse large B-cell lymphoma

KW - Immunophenotype

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