Immunomodulatory Effects in Mice of Polyinosinic-Polycytidylic Acid Complexed with Poly-L-lysine and Carboxymethylcellulose

James E Talmadge, Joanne Adams, Hamblin Phillips, Margaret Collins, Barbara Lenz, Mark Schneider, Erich Schlick, Ralf Ruffmann, Robert H. Wiltrout, Michael A. Chirigos

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In this report, we describe the immunomodulatory characteristics of poly(l,C)-LC, a synthetic, double-stranded nucleic acid polymer, polyinosinic-polycytidylic acid, that is complexed with poly-L-lysine and solubilized by the addition of carboxymethylcellulose. We consistently observed, both in vitro and in vivo, stimulation of macrophage cytotoxicity and augmentation of natural killer-cell activity by poly(l,C)-LC. This immunomodulator also increased the allogeneic mixed-lymphocyte response, without any blastogenic effect on responder cells cultured in the absence of allogeneic stimulator cells. Further, the addition of poly(l,C)-LC to an allogeneic mixed-lymphocyte tumor reaction did not stimulate the development of cytotoxic effector T-cells. Poly(l,C)-LC did, however, have adjuvant activity when admixed with irradiated tumor cells in the immunization of syngeneic mice. Unlike classic adjuvants, poly(l,C)-LC also enhanced the development of specific cytotoxic T-lymphocytes when it was injected either i.v. or i.p. in conjunction with a vaccine delivered at an intradermal site. The results indicate that poly(l,C)-LC has considerable potential as an immunotherapeutic agent, with the ability not only to induce macrophage and NK cell activation but also to stimulate specific cytotoxic T-lymphocytes.

Original languageEnglish (US)
Pages (from-to)1058-1065
Number of pages8
JournalCancer Research
Volume45
Issue number3
StatePublished - Mar 1 1985

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Poly C
Poly I-C
Carboxymethylcellulose Sodium
Lysine
Cytotoxic T-Lymphocytes
Natural Killer Cells
Macrophages
Mixed Lymphocyte Culture Test
Immunologic Factors
Nucleic Acids
Cultured Cells
Immunization
Neoplasms
Polymers
Vaccines
Lymphocytes
T-Lymphocytes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Talmadge, J. E., Adams, J., Phillips, H., Collins, M., Lenz, B., Schneider, M., ... Chirigos, M. A. (1985). Immunomodulatory Effects in Mice of Polyinosinic-Polycytidylic Acid Complexed with Poly-L-lysine and Carboxymethylcellulose. Cancer Research, 45(3), 1058-1065.

Immunomodulatory Effects in Mice of Polyinosinic-Polycytidylic Acid Complexed with Poly-L-lysine and Carboxymethylcellulose. / Talmadge, James E; Adams, Joanne; Phillips, Hamblin; Collins, Margaret; Lenz, Barbara; Schneider, Mark; Schlick, Erich; Ruffmann, Ralf; Wiltrout, Robert H.; Chirigos, Michael A.

In: Cancer Research, Vol. 45, No. 3, 01.03.1985, p. 1058-1065.

Research output: Contribution to journalArticle

Talmadge, JE, Adams, J, Phillips, H, Collins, M, Lenz, B, Schneider, M, Schlick, E, Ruffmann, R, Wiltrout, RH & Chirigos, MA 1985, 'Immunomodulatory Effects in Mice of Polyinosinic-Polycytidylic Acid Complexed with Poly-L-lysine and Carboxymethylcellulose', Cancer Research, vol. 45, no. 3, pp. 1058-1065.
Talmadge, James E ; Adams, Joanne ; Phillips, Hamblin ; Collins, Margaret ; Lenz, Barbara ; Schneider, Mark ; Schlick, Erich ; Ruffmann, Ralf ; Wiltrout, Robert H. ; Chirigos, Michael A. / Immunomodulatory Effects in Mice of Polyinosinic-Polycytidylic Acid Complexed with Poly-L-lysine and Carboxymethylcellulose. In: Cancer Research. 1985 ; Vol. 45, No. 3. pp. 1058-1065.
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