Immunomodulation of natural killer activity by polyribonucleotides

Robert H. Wiltrout, Raoul R. Salup, Theresa A. Twilley, James E Talmadge

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Several synthetic polyribonucleotides have been examined for the ability to augment natural killer (NK) activity and induce interferon (IFN) production. The results demonstrate that the complex of polyinosinic-polycytidylic acid and poly-L-lysine, which has been stabilized in carboxymethylcellulose [poly(I,C)-LC], polyadenylic-polyuridylic acid [poly(A)˙poly(U)], and a labile poly (I,C) compound with mismatched bases, designated poly(I)˙poly(C12U), all augment NK activity. However, poly(A)˙poly(U) and poly(I)˙poly(C12U) are less efficient augmentors of NK activity on a milligram per kilogram basis, than is poly(I,C)-LC. Similarly, poly(I,C)-LC induces more serum IFN following administration of 1, 10, or 100 μg/mouse (1,000-10,000 U/ml) than does either poly(A)˙poly(U) (0-25 U/ml) or poly(I)˙poly(C12U) (0-250 U/ml) at the same doses. Further studies with poly(I,C)-LC demonstrated that this molecule is an excellent augmentor of liver-associated NK activity. In fact, administration of poly(I,C)-LC resulted in higher NK levels in liver (63% lysis) than in spleen (44%) or blood (36%), and the augmented NK response was maintained in the liver for up to 13 days, whereas levels of NK activity in both blood and spleen returned to normal by days 3-6.

Original languageEnglish (US)
Pages (from-to)512-517
Number of pages6
JournalJournal of Biological Response Modifiers
Volume4
Issue number5
StatePublished - Jan 1 1985

Fingerprint

Polyribonucleotides
Immunomodulation
Poly A-U
Poly U
Interferons
Liver
Spleen
Poly I-C
Carboxymethylcellulose Sodium
Lysine
polyriboinosinic-polyribocytidylic acid
Serum

Keywords

  • Immunomodulation
  • Interferon
  • NK activity
  • Polyribonucleotides

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Cancer Research

Cite this

Immunomodulation of natural killer activity by polyribonucleotides. / Wiltrout, Robert H.; Salup, Raoul R.; Twilley, Theresa A.; Talmadge, James E.

In: Journal of Biological Response Modifiers, Vol. 4, No. 5, 01.01.1985, p. 512-517.

Research output: Contribution to journalArticle

Wiltrout, Robert H. ; Salup, Raoul R. ; Twilley, Theresa A. ; Talmadge, James E. / Immunomodulation of natural killer activity by polyribonucleotides. In: Journal of Biological Response Modifiers. 1985 ; Vol. 4, No. 5. pp. 512-517.
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