Immunological response to a Staphylococcus aureus fibronectin-binding protein

Pawel S Ciborowski, J. I. Flock, T. Wadstrom

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A protein (gal-FnBP), constructed by fusion of the genes encoding β-galactosidase of Escherichia coli and the binding domains of fibronectin-binding protein (FnBP) of Staphylococcus aureus was used. FnBP is a surface protein responsible for attachment of bacteria to extracellular matrix of various host tissues. Gal-FnBP is more stable and can be produced in larger quantities than native FnBP. The binding specificity of this fusion protein was established in a Western blot analysis. Treatment of gal-FnBP with formalin inactivated the binding capacity of the protein but immunogenicity was retained. Immunisation of mice with formalin-treated gal-FnBP resulted in high antibody titres against the fibronectin binding part of this fusion protein. These antibodies were measured by their ability to block the specific binding of fibronectin to gal-FnBP in a blocking assay. Sera raised against formalin-treated gal-FnBP and non-treated gal-FnBP blocked this binding to 40 and 25% respectively, thereby indicating the usefulness of gal-FnBP as a vaccine component.

Original languageEnglish (US)
Pages (from-to)376-381
Number of pages6
JournalJournal of Medical Microbiology
Volume37
Issue number6
DOIs
StatePublished - Jan 1 1992

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Fibronectins
Staphylococcus aureus
Carrier Proteins
Formaldehyde
Galactosidases
Proteins
Antibodies
Gene Fusion
Protein Binding
Extracellular Matrix
Immunization
Membrane Proteins
Vaccines
Western Blotting
Escherichia coli
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Immunological response to a Staphylococcus aureus fibronectin-binding protein. / Ciborowski, Pawel S; Flock, J. I.; Wadstrom, T.

In: Journal of Medical Microbiology, Vol. 37, No. 6, 01.01.1992, p. 376-381.

Research output: Contribution to journalArticle

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