Immunological probes for chromatin structure

M. Bustin, P. D. Kurth, E. N. Moudrianakis, D. Goldblatt, R. Sperling, William B Rizzo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Several methods for quantitating the reaction of the antihistone sera with purified chromatin and isolated nucleosomes have been devised. With these antisera it is possible to visualize the in situ organization of histones in nuclei, metaphase chromosomes and polytene chromosomes by the immunofluorescence technique. The organization of histone at the level of the single nucleosome in repressed, transcribed, and replicating regions of the genome can be resolved by immunoelectron microscopic techniques. In this paper the authors describe their studies on the in situ organization of histones in chromosomes and chromatin. In addition, the authors present evidence that, analogous to histone antibodies, lectins can be used as probes for chromatin structure.

Original languageEnglish (US)
Pages (from-to)379-388
Number of pages10
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume42
Issue number1
StatePublished - Dec 1 1977

Fingerprint

histones
Histones
Chromatin
chromatin
Chromosomes
nucleosomes
Nucleosomes
Polytene Chromosomes
chromosomes
polytene chromosomes
Metaphase
blood serum
metaphase
Lectins
lectins
fluorescent antibody technique
Fluorescent Antibody Technique
antiserum
Immune Sera
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Bustin, M., Kurth, P. D., Moudrianakis, E. N., Goldblatt, D., Sperling, R., & Rizzo, W. B. (1977). Immunological probes for chromatin structure. Cold Spring Harbor Symposia on Quantitative Biology, 42(1), 379-388.

Immunological probes for chromatin structure. / Bustin, M.; Kurth, P. D.; Moudrianakis, E. N.; Goldblatt, D.; Sperling, R.; Rizzo, William B.

In: Cold Spring Harbor Symposia on Quantitative Biology, Vol. 42, No. 1, 01.12.1977, p. 379-388.

Research output: Contribution to journalArticle

Bustin, M, Kurth, PD, Moudrianakis, EN, Goldblatt, D, Sperling, R & Rizzo, WB 1977, 'Immunological probes for chromatin structure', Cold Spring Harbor Symposia on Quantitative Biology, vol. 42, no. 1, pp. 379-388.
Bustin M, Kurth PD, Moudrianakis EN, Goldblatt D, Sperling R, Rizzo WB. Immunological probes for chromatin structure. Cold Spring Harbor Symposia on Quantitative Biology. 1977 Dec 1;42(1):379-388.
Bustin, M. ; Kurth, P. D. ; Moudrianakis, E. N. ; Goldblatt, D. ; Sperling, R. ; Rizzo, William B. / Immunological probes for chromatin structure. In: Cold Spring Harbor Symposia on Quantitative Biology. 1977 ; Vol. 42, No. 1. pp. 379-388.
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