Immunological comparison of the usual and atypical human serum cholinesterase phenotypes

Harry W. Eckerson, Allen Oseroff, Oksana Lockridge, Bert N. La Du

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Antiserum prepared against highly purified usual human serum cholinesterase (the most common phenotype) cross-reacted identically with the atypical serum cholinesterase. The level of circulating atypical enzyme protein, determined immunologically, was about 30% lower when the enzyme came from an atypical rather than a usual phenotype, and the level of enzyme activity measured enzymatically at Vmax with either o-nitrophenylbutyrate or benzoylcholine as substrate showed approximately the same degree of reduction. The average specific activity (activity at Vmax per microgram of enzyme protein) in sera from 28 usual and 20 atypical individuals did not differ significantly. These findings suggest that the atypical enzyme not only has altered catalytic properties (K)mbut also might be synthesized more slowly, or cleared in vivo more rapidly, than the usual enzyme.

Original languageEnglish (US)
Pages (from-to)93-108
Number of pages16
JournalBiochemical Genetics
Volume21
Issue number1-2
DOIs
StatePublished - Feb 1 1983

Fingerprint

cholinesterase
Cholinesterases
blood serum
phenotype
serum
enzyme
Phenotype
Enzymes
enzymes
Serum
Benzoylcholine
protein
Enzyme activity
blood proteins
enzyme activity
antiserum
Immune Sera
Proteins
comparison
Blood Proteins

Keywords

  • atypical cholinesterase
  • cholinesterase
  • human esterase
  • quantitive enzyme variation

ASJC Scopus subject areas

  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Immunological comparison of the usual and atypical human serum cholinesterase phenotypes. / Eckerson, Harry W.; Oseroff, Allen; Lockridge, Oksana; La Du, Bert N.

In: Biochemical Genetics, Vol. 21, No. 1-2, 01.02.1983, p. 93-108.

Research output: Contribution to journalArticle

Eckerson, Harry W. ; Oseroff, Allen ; Lockridge, Oksana ; La Du, Bert N. / Immunological comparison of the usual and atypical human serum cholinesterase phenotypes. In: Biochemical Genetics. 1983 ; Vol. 21, No. 1-2. pp. 93-108.
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