Immunologic indicators of clinical progression during canine Leishmania infantum infection

Paola M. Boggiatto, Amanda E. Ramer-Tait, Kyle Metz, Erin E. Kramer, Katherine Gibson-Corley, Kathleen Mullin, Jesse M. Hostetter, Jack M. Gallup, Douglas E. Jones, Christine A. Petersen

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

In both dogs and humans Leishmania infantum infection is more prevalent than disease, as infection often does not equate with clinical disease. Previous studies additively indicate that advanced clinical visceral leishmaniasis is characterized by increased production of anti-Leishmania antibodies, Leishmania-specific lymphoproliferative unresponsiveness, and decreased production of gamma interferon (IFN-γ) with a concomitant increase of interleukin-10 (IL-10). In order to differentiate infection versus progressive disease for better disease prognostication, we temporally evaluated humoral and cellular immunologic parameters of naturally infected dogs. The work presented here describes for the first time the temporal immune response to natural autochthonous L. infantum infection in foxhounds within the United States. Several key changes in immunological parameters should be considered when differentiating infection versus clinical disease, including a dramatic rise in IgG production, progressive increases in antigen-specific peripheral blood mononuclear cell proliferation, and IFN-γ production. Polysymptomatic disease is precluded by increased IL-10 production and consistent detection of parasite kinetoplast DNA in whole blood. This clinical presentation and the immuno-dysregulation mirror those observed in human patients, indicating that this animal model will be very useful for testing immunomodulatory anti-IL-10 and other therapies.

Original languageEnglish (US)
Pages (from-to)267-273
Number of pages7
JournalClinical and Vaccine Immunology
Volume17
Issue number2
DOIs
StatePublished - Feb 1 2010

Fingerprint

Leishmania infantum
Canidae
Infection
Interleukin-10
Blood
Kinetoplast DNA
Dogs
Visceral Leishmaniasis
Leishmania
Cell proliferation
Interferons
Interferon-gamma
Anti-Idiotypic Antibodies
Blood Cells
Parasites
Mirrors
Animals
Animal Models
Immunoglobulin G
Cell Proliferation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Boggiatto, P. M., Ramer-Tait, A. E., Metz, K., Kramer, E. E., Gibson-Corley, K., Mullin, K., ... Petersen, C. A. (2010). Immunologic indicators of clinical progression during canine Leishmania infantum infection. Clinical and Vaccine Immunology, 17(2), 267-273. https://doi.org/10.1128/CVI.00456-09

Immunologic indicators of clinical progression during canine Leishmania infantum infection. / Boggiatto, Paola M.; Ramer-Tait, Amanda E.; Metz, Kyle; Kramer, Erin E.; Gibson-Corley, Katherine; Mullin, Kathleen; Hostetter, Jesse M.; Gallup, Jack M.; Jones, Douglas E.; Petersen, Christine A.

In: Clinical and Vaccine Immunology, Vol. 17, No. 2, 01.02.2010, p. 267-273.

Research output: Contribution to journalArticle

Boggiatto, PM, Ramer-Tait, AE, Metz, K, Kramer, EE, Gibson-Corley, K, Mullin, K, Hostetter, JM, Gallup, JM, Jones, DE & Petersen, CA 2010, 'Immunologic indicators of clinical progression during canine Leishmania infantum infection', Clinical and Vaccine Immunology, vol. 17, no. 2, pp. 267-273. https://doi.org/10.1128/CVI.00456-09
Boggiatto, Paola M. ; Ramer-Tait, Amanda E. ; Metz, Kyle ; Kramer, Erin E. ; Gibson-Corley, Katherine ; Mullin, Kathleen ; Hostetter, Jesse M. ; Gallup, Jack M. ; Jones, Douglas E. ; Petersen, Christine A. / Immunologic indicators of clinical progression during canine Leishmania infantum infection. In: Clinical and Vaccine Immunology. 2010 ; Vol. 17, No. 2. pp. 267-273.
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