Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation

Ari Nowacek, Irena Kadiu, JoEllyn M McMillan, Howard Eliot Gendelman

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Cell-mediated nanoparticle delivery has recently emerged as an efficacious method of delivering therapeutic agents across physiological barriers. Use of cells as nanodelivery vehicles requires accurate assessment of their loading capacity and identification of intracellular compartments where nanoparticles are sequestered. This is of great interest since specific endocytic trafficking routes can ultimately influence the mode of nanoparticle release and their efficacy and function. Here, we describe a technique that allows for the isolation of individual populations of nanoparticle-containing endosomes for subsequent quantitative analysis and more accurate description of where nanoparticles are stored on a subcellular level.

Original languageEnglish (US)
Title of host publicationCellular and Subcellular Nanotechnology
Subtitle of host publicationMethods and Protocols
EditorsVolkmar Weissig, Mark Olsen, Tamer Elbayoumi
Pages41-46
Number of pages6
DOIs
StatePublished - May 8 2013

Publication series

NameMethods in Molecular Biology
Volume991
ISSN (Print)1064-3745

Fingerprint

Transport Vesicles
Nanoparticles
Pharmaceutical Preparations
Endosomes
Population

Keywords

  • Endosomes
  • Immunoisolation
  • Macrophage
  • Nanoparticles
  • Subcellular trafficking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nowacek, A., Kadiu, I., McMillan, J. M., & Gendelman, H. E. (2013). Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation. In V. Weissig, M. Olsen, & T. Elbayoumi (Eds.), Cellular and Subcellular Nanotechnology: Methods and Protocols (pp. 41-46). (Methods in Molecular Biology; Vol. 991). https://doi.org/10.1007/978-1-62703-336-7_5

Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation. / Nowacek, Ari; Kadiu, Irena; McMillan, JoEllyn M; Gendelman, Howard Eliot.

Cellular and Subcellular Nanotechnology: Methods and Protocols. ed. / Volkmar Weissig; Mark Olsen; Tamer Elbayoumi. 2013. p. 41-46 (Methods in Molecular Biology; Vol. 991).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nowacek, A, Kadiu, I, McMillan, JM & Gendelman, HE 2013, Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation. in V Weissig, M Olsen & T Elbayoumi (eds), Cellular and Subcellular Nanotechnology: Methods and Protocols. Methods in Molecular Biology, vol. 991, pp. 41-46. https://doi.org/10.1007/978-1-62703-336-7_5
Nowacek A, Kadiu I, McMillan JM, Gendelman HE. Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation. In Weissig V, Olsen M, Elbayoumi T, editors, Cellular and Subcellular Nanotechnology: Methods and Protocols. 2013. p. 41-46. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-336-7_5
Nowacek, Ari ; Kadiu, Irena ; McMillan, JoEllyn M ; Gendelman, Howard Eliot. / Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation. Cellular and Subcellular Nanotechnology: Methods and Protocols. editor / Volkmar Weissig ; Mark Olsen ; Tamer Elbayoumi. 2013. pp. 41-46 (Methods in Molecular Biology).
@inbook{04c800febc25456e8d7ceac5002dd704,
title = "Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation",
abstract = "Cell-mediated nanoparticle delivery has recently emerged as an efficacious method of delivering therapeutic agents across physiological barriers. Use of cells as nanodelivery vehicles requires accurate assessment of their loading capacity and identification of intracellular compartments where nanoparticles are sequestered. This is of great interest since specific endocytic trafficking routes can ultimately influence the mode of nanoparticle release and their efficacy and function. Here, we describe a technique that allows for the isolation of individual populations of nanoparticle-containing endosomes for subsequent quantitative analysis and more accurate description of where nanoparticles are stored on a subcellular level.",
keywords = "Endosomes, Immunoisolation, Macrophage, Nanoparticles, Subcellular trafficking",
author = "Ari Nowacek and Irena Kadiu and McMillan, {JoEllyn M} and Gendelman, {Howard Eliot}",
year = "2013",
month = "5",
day = "8",
doi = "10.1007/978-1-62703-336-7_5",
language = "English (US)",
isbn = "9781627033350",
series = "Methods in Molecular Biology",
pages = "41--46",
editor = "Volkmar Weissig and Mark Olsen and Tamer Elbayoumi",
booktitle = "Cellular and Subcellular Nanotechnology",

}

TY - CHAP

T1 - Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation

AU - Nowacek, Ari

AU - Kadiu, Irena

AU - McMillan, JoEllyn M

AU - Gendelman, Howard Eliot

PY - 2013/5/8

Y1 - 2013/5/8

N2 - Cell-mediated nanoparticle delivery has recently emerged as an efficacious method of delivering therapeutic agents across physiological barriers. Use of cells as nanodelivery vehicles requires accurate assessment of their loading capacity and identification of intracellular compartments where nanoparticles are sequestered. This is of great interest since specific endocytic trafficking routes can ultimately influence the mode of nanoparticle release and their efficacy and function. Here, we describe a technique that allows for the isolation of individual populations of nanoparticle-containing endosomes for subsequent quantitative analysis and more accurate description of where nanoparticles are stored on a subcellular level.

AB - Cell-mediated nanoparticle delivery has recently emerged as an efficacious method of delivering therapeutic agents across physiological barriers. Use of cells as nanodelivery vehicles requires accurate assessment of their loading capacity and identification of intracellular compartments where nanoparticles are sequestered. This is of great interest since specific endocytic trafficking routes can ultimately influence the mode of nanoparticle release and their efficacy and function. Here, we describe a technique that allows for the isolation of individual populations of nanoparticle-containing endosomes for subsequent quantitative analysis and more accurate description of where nanoparticles are stored on a subcellular level.

KW - Endosomes

KW - Immunoisolation

KW - Macrophage

KW - Nanoparticles

KW - Subcellular trafficking

UR - http://www.scopus.com/inward/record.url?scp=84877045690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877045690&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-336-7_5

DO - 10.1007/978-1-62703-336-7_5

M3 - Chapter

C2 - 23546657

AN - SCOPUS:84877045690

SN - 9781627033350

T3 - Methods in Molecular Biology

SP - 41

EP - 46

BT - Cellular and Subcellular Nanotechnology

A2 - Weissig, Volkmar

A2 - Olsen, Mark

A2 - Elbayoumi, Tamer

ER -