Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation

Ari Nowacek, Irena Kadiu, JoEllyn M McMillan, Howard Eliot Gendelman

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Cell-mediated nanoparticle delivery has recently emerged as an efficacious method of delivering therapeutic agents across physiological barriers. Use of cells as nanodelivery vehicles requires accurate assessment of their loading capacity and identification of intracellular compartments where nanoparticles are sequestered. This is of great interest since specific endocytic trafficking routes can ultimately influence the mode of nanoparticle release and their efficacy and function. Here, we describe a technique that allows for the isolation of individual populations of nanoparticle-containing endosomes for subsequent quantitative analysis and more accurate description of where nanoparticles are stored on a subcellular level.

Original languageEnglish (US)
Title of host publicationCellular and Subcellular Nanotechnology
Subtitle of host publicationMethods and Protocols
EditorsVolkmar Weissig, Mark Olsen, Tamer Elbayoumi
Pages41-46
Number of pages6
DOIs
Publication statusPublished - May 8 2013

Publication series

NameMethods in Molecular Biology
Volume991
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Endosomes
  • Immunoisolation
  • Macrophage
  • Nanoparticles
  • Subcellular trafficking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nowacek, A., Kadiu, I., McMillan, J. M., & Gendelman, H. E. (2013). Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation. In V. Weissig, M. Olsen, & T. Elbayoumi (Eds.), Cellular and Subcellular Nanotechnology: Methods and Protocols (pp. 41-46). (Methods in Molecular Biology; Vol. 991). https://doi.org/10.1007/978-1-62703-336-7_5