Immunohistological characterization of proximal colonic lymphoid tissue in the rat

N. K. De Boer, F. G M Kroese, John G Sharp, G. A. Perry

Research output: Contribution to journalArticle

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Abstract

Proximal colonic lymphoid tissue (PCLT) is a lymphoid structure located in the proximal colon of the mouse and the rat. In the present investigation we studied the immunomorphology and cytology of PCLT in the rat. We also studied sites of lymphocyte proliferation using the BrdU‐anti BrdU technique. Results demonstrated no evident phenotypical differences between the lymphocyte populations of PCLT and either jejunal or ileal Peyer's patches (PP). The majority of the lymphocytes within PCLT were B cells localized in follicles, which were separated from each other by interfollicular T cell areas. Germinal centers (GC), containing ED5+ follicular dendritic cells, are found within PCLT follicles. The T cell areas contained both MHC Class II+ interdigitating cells and high endothelial venules. Studies using BrdU‐anti BrdU indicated that lymphocyte proliferation within PCLT taken place mainly in germinal centers. Together the data show that the organization, lymphoid constituents, and sites of lymphocyte production are very similar in PCLT and PP. We therefore conclude that PCLT in the rats is not a Bursa equivalent, but more likely a PP with some special characteristics. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)569-576
Number of pages8
JournalThe Anatomical Record
Volume233
Issue number4
DOIs
StatePublished - Aug 1992

Fingerprint

Lymphoid Tissue
rats
Peyer's Patches
Peyer's patches
Lymphocytes
Germinal Center
lymphocytes
Bromodeoxyuridine
lymphocyte proliferation
T-lymphocytes
Follicular Dendritic Cells
cytology
T-Lymphocytes
Venules
tissues
tissue
dendritic cells
cell biology
Dendritic Cells
B-lymphocytes

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Immunohistological characterization of proximal colonic lymphoid tissue in the rat. / De Boer, N. K.; Kroese, F. G M; Sharp, John G; Perry, G. A.

In: The Anatomical Record, Vol. 233, No. 4, 08.1992, p. 569-576.

Research output: Contribution to journalArticle

De Boer, N. K. ; Kroese, F. G M ; Sharp, John G ; Perry, G. A. / Immunohistological characterization of proximal colonic lymphoid tissue in the rat. In: The Anatomical Record. 1992 ; Vol. 233, No. 4. pp. 569-576.
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