Immunohistochemistry of Pneumocystis carinii infection.

Stanley J Radio, S. Hansen, J. Goldsmith, James Linder

Research output: Contribution to journalArticle

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Abstract

Pneumocystis carinii is the pre-eminent pulmonary pathogen and leading cause of death in patients with acquired immunodeficiency syndrome (AIDS). The diagnosis of this organism depends upon the morphologic demonstration of the cyst wall, trophozoite, or sporozoite in specimens from the lower respiratory tract. A variety of histochemical stains have been used to identify P. carinii, each with considerable limitations in specificity. Extrapulmonary spread and unusually destructive pulmonary patterns associated with P. carinii, although once considered rare, are seen occasionally in patients with AIDS. Traditional stains have proven to be less reliable in extrapulmonary sites. In 13 patients with AIDS, we stained formalin-fixed paraffin-embedded autopsy lung and other visceral organ sections using monoclonal antibody 3F6 (Dako, Santa Barbara, CA) to P. carinii. The antibody stained P. carinii in the lungs of seven patients with P. carinii pneumonia by Gomori methenamine silver stain (GMS). Numerous aggregates of P. carinii cysts were marked within alveoli, as is usually seen with other stains. No antibody staining was present in autopsy lung sections from non-AIDS patients with viral, fungal, or bacterial pneumonia. Clinically occult extrapulmonary P. carinii infection was seen in 4/7 (57%) patients with P. carinii pneumonia at autopsy. Monoclonal antibody to P. carinii stained organisms in all four (100%) patients with disseminated disease, as compared with 1/4 (25%) staining with GMS. Antibody staining of P. carinii was demonstrated in sections of thyroid, adrenal, and carinal lymph node (one patient), esophagus (one patient), kidney (one patient), and heart, thyroid, kidney, adrenal, liver, stomach, pancreas, spleen, and bone marrow (one patient).(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)462-469
Number of pages8
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume3
Issue number4
StatePublished - Jul 1 1990

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Pneumocystis Infections
Pneumocystis carinii
Immunohistochemistry
Coloring Agents
Lung
Methenamine
Autopsy
Acquired Immunodeficiency Syndrome
Pneumocystis Pneumonia
Staining and Labeling
Antibodies
Cysts
Thyroid Gland
Monoclonal Antibodies
Viral Pneumonia
Kidney
Bacterial Pneumonia
Sporozoites
Trophozoites
Respiratory System

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistochemistry of Pneumocystis carinii infection. / Radio, Stanley J; Hansen, S.; Goldsmith, J.; Linder, James.

In: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 3, No. 4, 01.07.1990, p. 462-469.

Research output: Contribution to journalArticle

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