Immunohistochemical localization of luteinizing hormone‐releasing hormone (LHRH) in the hypothalamus of adult female hamsters treated neonatally with monosodium glutamate or hypertonic saline

Albert A. Lamperti, Gary E. Pickard

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4 Citations (Scopus)

Abstract

The immunohistochemical localization of luteinizing hormone‐releasing hormone (LHRH) was studied in paraffin and vibratome‐sectioned tissue from adult female hamsters that were treated neonatally with monosodium glutamate (MSG) or hypertonic saline. There appeared to be a reduction in LHRH‐positive fibers in the median eminence of animals with an MSG‐induced lesion of the arcuate nucleus in paraffin‐embedded tissue. However, when unembedded tissue was cut on a vibratome, the distribution of LHRH‐positive fibers and perikarya was similar in both groups of animals. Fibers were seen coursing through the periventricular area and lateral hypo‐thalamus to the median eminence. In addition, LHRH‐positive fibers were seen in the organum vasculosum of the lamina terminalis, subfornical organ, septal and preoptic areas, fasciculus retroflexus, habenular complex, and several regions in the basal forebrain. Animals that were pretreated with colchicine had LHRH‐positive perikarya in the medial habenular nucleus, diagonal band of Broca, and the medial olfactory tract.

Original languageEnglish (US)
Pages (from-to)131-141
Number of pages11
JournalThe Anatomical Record
Volume209
Issue number1
DOIs
StatePublished - May 1984

Fingerprint

monosodium glutamate
Sodium Glutamate
luteinizing hormone
hypothalamus
Luteinizing Hormone
hamsters
Cricetinae
Hypothalamus
hormone
Median Eminence
Diagonal Band of Broca
Subfornical Organ
Lateral Hypothalamic Area
Habenula
Septum of Brain
preoptic area
animal
Septal Nuclei
animals
Arcuate Nucleus of Hypothalamus

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

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title = "Immunohistochemical localization of luteinizing hormone‐releasing hormone (LHRH) in the hypothalamus of adult female hamsters treated neonatally with monosodium glutamate or hypertonic saline",
abstract = "The immunohistochemical localization of luteinizing hormone‐releasing hormone (LHRH) was studied in paraffin and vibratome‐sectioned tissue from adult female hamsters that were treated neonatally with monosodium glutamate (MSG) or hypertonic saline. There appeared to be a reduction in LHRH‐positive fibers in the median eminence of animals with an MSG‐induced lesion of the arcuate nucleus in paraffin‐embedded tissue. However, when unembedded tissue was cut on a vibratome, the distribution of LHRH‐positive fibers and perikarya was similar in both groups of animals. Fibers were seen coursing through the periventricular area and lateral hypo‐thalamus to the median eminence. In addition, LHRH‐positive fibers were seen in the organum vasculosum of the lamina terminalis, subfornical organ, septal and preoptic areas, fasciculus retroflexus, habenular complex, and several regions in the basal forebrain. Animals that were pretreated with colchicine had LHRH‐positive perikarya in the medial habenular nucleus, diagonal band of Broca, and the medial olfactory tract.",
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AU - Pickard, Gary E.

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N2 - The immunohistochemical localization of luteinizing hormone‐releasing hormone (LHRH) was studied in paraffin and vibratome‐sectioned tissue from adult female hamsters that were treated neonatally with monosodium glutamate (MSG) or hypertonic saline. There appeared to be a reduction in LHRH‐positive fibers in the median eminence of animals with an MSG‐induced lesion of the arcuate nucleus in paraffin‐embedded tissue. However, when unembedded tissue was cut on a vibratome, the distribution of LHRH‐positive fibers and perikarya was similar in both groups of animals. Fibers were seen coursing through the periventricular area and lateral hypo‐thalamus to the median eminence. In addition, LHRH‐positive fibers were seen in the organum vasculosum of the lamina terminalis, subfornical organ, septal and preoptic areas, fasciculus retroflexus, habenular complex, and several regions in the basal forebrain. Animals that were pretreated with colchicine had LHRH‐positive perikarya in the medial habenular nucleus, diagonal band of Broca, and the medial olfactory tract.

AB - The immunohistochemical localization of luteinizing hormone‐releasing hormone (LHRH) was studied in paraffin and vibratome‐sectioned tissue from adult female hamsters that were treated neonatally with monosodium glutamate (MSG) or hypertonic saline. There appeared to be a reduction in LHRH‐positive fibers in the median eminence of animals with an MSG‐induced lesion of the arcuate nucleus in paraffin‐embedded tissue. However, when unembedded tissue was cut on a vibratome, the distribution of LHRH‐positive fibers and perikarya was similar in both groups of animals. Fibers were seen coursing through the periventricular area and lateral hypo‐thalamus to the median eminence. In addition, LHRH‐positive fibers were seen in the organum vasculosum of the lamina terminalis, subfornical organ, septal and preoptic areas, fasciculus retroflexus, habenular complex, and several regions in the basal forebrain. Animals that were pretreated with colchicine had LHRH‐positive perikarya in the medial habenular nucleus, diagonal band of Broca, and the medial olfactory tract.

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