Immunohistochemical localization of basement membrane collagens and associated proteins in the murine cochlea

Dominic E Cosgrove, Gina Samuelson, Jeff Pinnt

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Immunohistochemistry using antibodies specific for each of the basement membrane collagen chains was used to assess the location and composition of basement membranes in the mouse cochlea. The classical chains (COL4A1, 4A2) localized primarily in the osseous spiral lamina and in the capillaries of the spiral ligament. In contrast, the novel collagen chains (4A3, 4A4, and 4A5) localized to the interdental cells of the sulcus, the inner sulcus, the basilar membrane, and the region of type II fibrocytes in the spiral ligament. Antibodies against type 4A5 collagen also heavily stained the stria vascularis. Weak staining in the stria was observed with antibodies against 4A3. Basement membrane-associated proteins were also assessed. The basement membrane in the perineurium of the osseous spiral lamina immunostained using antibodies against laminin, heparan sulfate proteoglycan, and entactin. The basilar membrane contained only fibronectin in association with the novel collagen chains. The capillaries of the spiral ligament and the stria vascularis stained heavily for heparin sulfate proteoglycan and laminin. Generalized staining for laminin was observed in the spiral ligament. These results indicate that an abundance of basement membrane collagen containing extracellular matrix exists in the murine cochlea and that the composition of these matrices are surprisingly varied and tissue specific.

Original languageEnglish (US)
Pages (from-to)54-65
Number of pages12
JournalHearing Research
Volume97
Issue number1-2
DOIs
StatePublished - Aug 1 1996

Fingerprint

Spiral Ligament of Cochlea
Cochlea
Basement Membrane
Membrane Proteins
Collagen
Spiral Lamina
Laminin
Basilar Membrane
Stria Vascularis
Antibodies
Staining and Labeling
Heparan Sulfate Proteoglycans
Peripheral Nerves
Fibronectins
Extracellular Matrix
Immunohistochemistry

Keywords

  • Basement membrane
  • Cochlea
  • Immunohistology

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Immunohistochemical localization of basement membrane collagens and associated proteins in the murine cochlea. / Cosgrove, Dominic E; Samuelson, Gina; Pinnt, Jeff.

In: Hearing Research, Vol. 97, No. 1-2, 01.08.1996, p. 54-65.

Research output: Contribution to journalArticle

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