Immunohistochemical characterization of lymphocytes in uninflamed ventricular myocardium

Implication for myocarditis

James Linder, R. S. Cassling, W. C. Rogler, J. E. Wilson, Rodney Smith Markin, Thomas Delbert Sears, B. M. McManus

Research output: Contribution to journalArticle

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Abstract

Evaluation of endomyocardial biopsy specimens for lymphocytic myocarditis requires accurate identification of lymphocytes, a task at times difficult considering that other myocardial interstitial cells mimic lymphocyte morphology. To wit, the number of mononuclear cells present in normal (uninflamed) myocardium has remained in doubt. We studied the myocardium from hearts that were obtained at autopsy and transvenous endomyocardial biopsy specimens with monoclonal antibodies and immunohistochemical stains to determine the normal numbers and distribution of lymphocytes in uninflamed hearts. In the ventricular myocardium of hearts obtained at autopsy, total immunohistochemically marked lymphocytes averaged 3.6/sq mm, with most being T-cell marker-positive. The ratio of T-helper to T-suppressor-cytotoxic (OKT-4:OKT-8) cells was 1.44. The number of myocardial lymphocytes demonstrated by immunohistochemical staining correlated well with, but was consistently less than, the number obtained by quantitative light microscopic studies on unmarked samples. Thus, the immunohistochemical technique allows for objective enumeration of cells and provides avenues for quantitation of lymphocyte subpopulations in inflamed hearts.

Original languageEnglish (US)
Pages (from-to)917-920
Number of pages4
JournalArchives of Pathology and Laboratory Medicine
Volume109
Issue number10
StatePublished - Dec 1 1985

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Myocarditis
Myocardium
Lymphocytes
Lymphocyte Count
Autopsy
Biopsy
Wit and Humor
Normal Distribution
Lymphocyte Subsets
Coloring Agents
Cell Count
Monoclonal Antibodies
Staining and Labeling
T-Lymphocytes
Light

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Immunohistochemical characterization of lymphocytes in uninflamed ventricular myocardium : Implication for myocarditis. / Linder, James; Cassling, R. S.; Rogler, W. C.; Wilson, J. E.; Markin, Rodney Smith; Sears, Thomas Delbert; McManus, B. M.

In: Archives of Pathology and Laboratory Medicine, Vol. 109, No. 10, 01.12.1985, p. 917-920.

Research output: Contribution to journalArticle

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AU - McManus, B. M.

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AB - Evaluation of endomyocardial biopsy specimens for lymphocytic myocarditis requires accurate identification of lymphocytes, a task at times difficult considering that other myocardial interstitial cells mimic lymphocyte morphology. To wit, the number of mononuclear cells present in normal (uninflamed) myocardium has remained in doubt. We studied the myocardium from hearts that were obtained at autopsy and transvenous endomyocardial biopsy specimens with monoclonal antibodies and immunohistochemical stains to determine the normal numbers and distribution of lymphocytes in uninflamed hearts. In the ventricular myocardium of hearts obtained at autopsy, total immunohistochemically marked lymphocytes averaged 3.6/sq mm, with most being T-cell marker-positive. The ratio of T-helper to T-suppressor-cytotoxic (OKT-4:OKT-8) cells was 1.44. The number of myocardial lymphocytes demonstrated by immunohistochemical staining correlated well with, but was consistently less than, the number obtained by quantitative light microscopic studies on unmarked samples. Thus, the immunohistochemical technique allows for objective enumeration of cells and provides avenues for quantitation of lymphocyte subpopulations in inflamed hearts.

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