Immunoglobulin analysis tool

A novel tool for the analysis of human and mouse heavy and light chain transcripts

Tobias Rogosch, Sebastian Kerzel, Kam Hon Hoi, Zhixin Zhang, Rolf F. Maier, Gregory C. Ippolito, Michael Zemlin

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Sequence analysis of immunoglobulin (Ig) heavy and light chain transcripts can refine categorization of B cell subpopulations and can shed light on the selective forces that act during immune responses or immune dysregulation, such as autoimmunity, allergy, and B cell malignancy. High-throughput sequencing yields Ig transcript collections of unprecedented size. The authoritative web-based IMGT/HighV-QUEST program is capable of analyzing large collections of transcripts and provides annotated output files to describe many key properties of Ig transcripts. However, additional processing of these flat files is required to create figures, or to facilitate analysis of additional features and comparisons between sequence sets. We present an easy-to-use Microsoft® Excel® based software, named Immunoglobulin AnalysisTool (IgAT), for the summary, interrogation, and further processing of IMGT/HighV-QUEST output files. IgAT generates descriptive statistics and high-quality figures for collections of murine or human Ig heavy or light chain transcripts ranging from 1 to 150,000 sequences. In addition to traditionally studied properties of Ig transcripts - such as the usage of germline gene segments, or the length and composition of the CDR-3 region - IgAT also uses published algorithms to calculate the probability of antigen selection based on somatic mutational patterns, the average hydrophobicity of the antigenbinding sites, and predictable structural properties of the CDR-H3 loop according to Shirai's H3-rules. These refined analyses provide in-depth information about the selective forces acting upon Ig repertoires and allowthe statistical and graphical comparison of two or more sequence sets. IgAT is easy to use on any computer running Excel® 2003 or higher. Thus, IgAT is a useful tool to gain insights into the selective forces and functional properties of small to extremely large collections of Ig transcripts, thereby assisting a researcher to mine a data set to its fullest.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalFrontiers in immunology
Volume3
Issue numberJUN
DOIs
StatePublished - Dec 1 2012

Fingerprint

Immunoglobulins
Light
Immunoglobulin Light Chains
Immunoglobulin Heavy Chains
B-Lymphocytes
Autoimmunity
Hydrophobic and Hydrophilic Interactions
Sequence Analysis
Hypersensitivity
Software
Research Personnel
Antigens
Genes

Keywords

  • Antibody repertoire
  • Deep sequencing
  • High-throughput analysis
  • Immunoglobulin heavy chain gene
  • Immunoglobulin light chain gene
  • Rearrangement
  • Sequence analysis software
  • Somatic mutation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Rogosch, T., Kerzel, S., Hoi, K. H., Zhang, Z., Maier, R. F., Ippolito, G. C., & Zemlin, M. (2012). Immunoglobulin analysis tool: A novel tool for the analysis of human and mouse heavy and light chain transcripts. Frontiers in immunology, 3(JUN), 1-14. https://doi.org/10.3389/fimmu.2012.00176

Immunoglobulin analysis tool : A novel tool for the analysis of human and mouse heavy and light chain transcripts. / Rogosch, Tobias; Kerzel, Sebastian; Hoi, Kam Hon; Zhang, Zhixin; Maier, Rolf F.; Ippolito, Gregory C.; Zemlin, Michael.

In: Frontiers in immunology, Vol. 3, No. JUN, 01.12.2012, p. 1-14.

Research output: Contribution to journalArticle

Rogosch, T, Kerzel, S, Hoi, KH, Zhang, Z, Maier, RF, Ippolito, GC & Zemlin, M 2012, 'Immunoglobulin analysis tool: A novel tool for the analysis of human and mouse heavy and light chain transcripts', Frontiers in immunology, vol. 3, no. JUN, pp. 1-14. https://doi.org/10.3389/fimmu.2012.00176
Rogosch, Tobias ; Kerzel, Sebastian ; Hoi, Kam Hon ; Zhang, Zhixin ; Maier, Rolf F. ; Ippolito, Gregory C. ; Zemlin, Michael. / Immunoglobulin analysis tool : A novel tool for the analysis of human and mouse heavy and light chain transcripts. In: Frontiers in immunology. 2012 ; Vol. 3, No. JUN. pp. 1-14.
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