Immunofluorescence localization of vinculin in ectoplasmic (“junctional”) specializations of rat sertoli cells

B. D. Grove, D. C. Pfeiffer, S. Allen, A. W. Vogl

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We have investigated, using indirect immunofluorescence techniques, the possibility that vinculin is a component of Sertoli cell ectoplasmic specializations. Affinity‐purified polyclonal antibodies produced against human platelet vinculin were used to probe fixed frozen sections of rat testis. Specific fluorescence occurs in Sertoli cell regions adjacent to spermatids and to basally situated junctional complexes, sites at which ectoplasmic specializations are known to occur. Staining also occurs in Sertoli cell regions associated with tubulobulbar complexes. The antibody also labels focal contacts in cultured human dermal fibroblasts, apical junctional sites of rat epididymal epithelium, and dense plaques of smooth muscle. Our results are consistent with the prediction that vinculin is likely a component of ectoplasmic specializations and are also consistent with the hypothesis that these structures are a form of actin‐associated adhesion complex.

Original languageEnglish (US)
Pages (from-to)44-56
Number of pages13
JournalAmerican Journal of Anatomy
Volume188
Issue number1
DOIs
StatePublished - May 1990

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Vinculin
Sertoli Cells
Fluorescent Antibody Technique
Focal Adhesions
Spermatids
Antibodies
Frozen Sections
Indirect Fluorescent Antibody Technique
Smooth Muscle
Testis
Blood Platelets
Epithelium
Fibroblasts
Fluorescence
Staining and Labeling
Skin

ASJC Scopus subject areas

  • Anatomy

Cite this

Immunofluorescence localization of vinculin in ectoplasmic (“junctional”) specializations of rat sertoli cells. / Grove, B. D.; Pfeiffer, D. C.; Allen, S.; Vogl, A. W.

In: American Journal of Anatomy, Vol. 188, No. 1, 05.1990, p. 44-56.

Research output: Contribution to journalArticle

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