Immunochemical identification of a tRNA-independent cytokinin-like compound in Salmonella typhimurium

Paul H Blum, Bruce N. Ames

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chemical and immunological characterization of Salmonella typhimurium cell extracts indicates that this organism produces a molecule which closely resembles the plant growth regulator, cytokinin. Alcohol-soluble cationic ultraviolet-absorbing material was fractionated by reverse-phase HPLC using gradient conditions optimized previously for modified nucleoside separation. A single hydrophobic compound was identified in the cytokinin region of the gradient, and limited quantities of the compound were prepared by HPLC fractionation of crude extracts. The compound demonstrated significant activity in a radioimmunoassay for cytokinins which detects N6-isopentenylated adenine derivatives. Boronate affinity chromatography indicated the compound is likely to be ribosylated and therefore a nucleoside. These and other tests indicate the compound has the most notable structural characteristics of a cytokinin. Spectral analysis and chromatographic comparison with cytokinin standards indicate the compound also has some unique structural features. Presence of the compound in extracts of an S. typhimurium mutant blocked for synthesis of tRNA-derived cytokinins excluded tRNA as a source for the compound and implicates existence of a tRNA-independent pathway for cytokinin biosynthesis in this bacterial species.

Original languageEnglish (US)
Pages (from-to)196-202
Number of pages7
JournalBBA - Gene Structure and Expression
Volume1007
Issue number2
DOIs
StatePublished - Mar 1 1989

Fingerprint

Cytokinins
Salmonella
Salmonella typhimurium
Transfer RNA
Nucleosides
High Pressure Liquid Chromatography
Affinity chromatography
Plant Growth Regulators
Biosynthesis
Adenine
Fractionation
Cell Extracts
Complex Mixtures
Affinity Chromatography
Spectrum analysis
Radioimmunoassay
Alcohols
Derivatives
Molecules

Keywords

  • (S. typhimurium (miaA))
  • Boronate chromatography
  • Cytokinin
  • HPLC
  • Radioimmunoassay
  • miaA
  • tRNA independent cytokinin-like compound

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Immunochemical identification of a tRNA-independent cytokinin-like compound in Salmonella typhimurium. / Blum, Paul H; Ames, Bruce N.

In: BBA - Gene Structure and Expression, Vol. 1007, No. 2, 01.03.1989, p. 196-202.

Research output: Contribution to journalArticle

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