Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay

Jesseeca Obenoskey, Dakota R. Lane, Audrey L Atkin, Bessie W. Kebaara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The nonsense-mediated mRNA decay (NMD) pathway is a specialized pathway that triggers the rapid degradation of select mRNAs. Initially, identified as a pathway that degrades mRNAs with premature termination codons, NMD is now recognized as a pathway that also regulates some natural mRNAs. Since natural mRNAs do not typically contain premature termination codons, these mRNAs contain features that target them to NMD. In Saccharomyces cerevisiae mRNAs with atypically long 3'-UTRs are usually degraded by NMD, however in some conditions a constitutively expressed SSY5 mRNA with multiple NMD targeting signals including an atypically long 3'-UTR is an exception. We investigated the features of the SSY5 mRNAs that confer immunity to NMD. We found that the SSY5 mRNA 3'-UTRs are sufficient to target NMD insensitive mRNA to the pathway. Replacing the SSY5 3'-UTRs with the cyc1-512 3'-UTRs, known to target mRNAs to NMD or with the CYC1 3'-UTR, known not to target mRNAs to NMD, resulted in production of SSY5 mRNAs that were regulated by NMD. These observations suggest that the SSY5 mRNAs require sequences both within the 5'-UTR and/or ORF as well as the 3'-UTR to escape decay by NMD.

Original languageEnglish (US)
Article number25
JournalFrontiers in Molecular Biosciences
Volume1
Issue numberDEC
DOIs
StatePublished - Dec 8 2014

Fingerprint

Nonsense Mediated mRNA Decay
Yeast
Saccharomyces cerevisiae
Immunity
Messenger RNA
3' Untranslated Regions
Nonsense Codon
5' Untranslated Regions
RNA Stability

Keywords

  • 3'-untranslated regions
  • MRNA decay
  • MRNA stability
  • Nonsense-mediated mRNA decay
  • SSY5

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay. / Obenoskey, Jesseeca; Lane, Dakota R.; Atkin, Audrey L; Kebaara, Bessie W.

In: Frontiers in Molecular Biosciences, Vol. 1, No. DEC, 25, 08.12.2014.

Research output: Contribution to journalArticle

Obenoskey, Jesseeca ; Lane, Dakota R. ; Atkin, Audrey L ; Kebaara, Bessie W. / Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay. In: Frontiers in Molecular Biosciences. 2014 ; Vol. 1, No. DEC.
@article{63685911c93f40b789c62c4a3ffc38dc,
title = "Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay",
abstract = "The nonsense-mediated mRNA decay (NMD) pathway is a specialized pathway that triggers the rapid degradation of select mRNAs. Initially, identified as a pathway that degrades mRNAs with premature termination codons, NMD is now recognized as a pathway that also regulates some natural mRNAs. Since natural mRNAs do not typically contain premature termination codons, these mRNAs contain features that target them to NMD. In Saccharomyces cerevisiae mRNAs with atypically long 3'-UTRs are usually degraded by NMD, however in some conditions a constitutively expressed SSY5 mRNA with multiple NMD targeting signals including an atypically long 3'-UTR is an exception. We investigated the features of the SSY5 mRNAs that confer immunity to NMD. We found that the SSY5 mRNA 3'-UTRs are sufficient to target NMD insensitive mRNA to the pathway. Replacing the SSY5 3'-UTRs with the cyc1-512 3'-UTRs, known to target mRNAs to NMD or with the CYC1 3'-UTR, known not to target mRNAs to NMD, resulted in production of SSY5 mRNAs that were regulated by NMD. These observations suggest that the SSY5 mRNAs require sequences both within the 5'-UTR and/or ORF as well as the 3'-UTR to escape decay by NMD.",
keywords = "3'-untranslated regions, MRNA decay, MRNA stability, Nonsense-mediated mRNA decay, SSY5",
author = "Jesseeca Obenoskey and Lane, {Dakota R.} and Atkin, {Audrey L} and Kebaara, {Bessie W.}",
year = "2014",
month = "12",
day = "8",
doi = "10.3389/fmolb.2014.00025",
language = "English (US)",
volume = "1",
journal = "Frontiers in Molecular Biosciences",
issn = "2296-889X",
publisher = "Frontiers Media S. A.",
number = "DEC",

}

TY - JOUR

T1 - Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay

AU - Obenoskey, Jesseeca

AU - Lane, Dakota R.

AU - Atkin, Audrey L

AU - Kebaara, Bessie W.

PY - 2014/12/8

Y1 - 2014/12/8

N2 - The nonsense-mediated mRNA decay (NMD) pathway is a specialized pathway that triggers the rapid degradation of select mRNAs. Initially, identified as a pathway that degrades mRNAs with premature termination codons, NMD is now recognized as a pathway that also regulates some natural mRNAs. Since natural mRNAs do not typically contain premature termination codons, these mRNAs contain features that target them to NMD. In Saccharomyces cerevisiae mRNAs with atypically long 3'-UTRs are usually degraded by NMD, however in some conditions a constitutively expressed SSY5 mRNA with multiple NMD targeting signals including an atypically long 3'-UTR is an exception. We investigated the features of the SSY5 mRNAs that confer immunity to NMD. We found that the SSY5 mRNA 3'-UTRs are sufficient to target NMD insensitive mRNA to the pathway. Replacing the SSY5 3'-UTRs with the cyc1-512 3'-UTRs, known to target mRNAs to NMD or with the CYC1 3'-UTR, known not to target mRNAs to NMD, resulted in production of SSY5 mRNAs that were regulated by NMD. These observations suggest that the SSY5 mRNAs require sequences both within the 5'-UTR and/or ORF as well as the 3'-UTR to escape decay by NMD.

AB - The nonsense-mediated mRNA decay (NMD) pathway is a specialized pathway that triggers the rapid degradation of select mRNAs. Initially, identified as a pathway that degrades mRNAs with premature termination codons, NMD is now recognized as a pathway that also regulates some natural mRNAs. Since natural mRNAs do not typically contain premature termination codons, these mRNAs contain features that target them to NMD. In Saccharomyces cerevisiae mRNAs with atypically long 3'-UTRs are usually degraded by NMD, however in some conditions a constitutively expressed SSY5 mRNA with multiple NMD targeting signals including an atypically long 3'-UTR is an exception. We investigated the features of the SSY5 mRNAs that confer immunity to NMD. We found that the SSY5 mRNA 3'-UTRs are sufficient to target NMD insensitive mRNA to the pathway. Replacing the SSY5 3'-UTRs with the cyc1-512 3'-UTRs, known to target mRNAs to NMD or with the CYC1 3'-UTR, known not to target mRNAs to NMD, resulted in production of SSY5 mRNAs that were regulated by NMD. These observations suggest that the SSY5 mRNAs require sequences both within the 5'-UTR and/or ORF as well as the 3'-UTR to escape decay by NMD.

KW - 3'-untranslated regions

KW - MRNA decay

KW - MRNA stability

KW - Nonsense-mediated mRNA decay

KW - SSY5

UR - http://www.scopus.com/inward/record.url?scp=84951775989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84951775989&partnerID=8YFLogxK

U2 - 10.3389/fmolb.2014.00025

DO - 10.3389/fmolb.2014.00025

M3 - Article

VL - 1

JO - Frontiers in Molecular Biosciences

JF - Frontiers in Molecular Biosciences

SN - 2296-889X

IS - DEC

M1 - 25

ER -