Immune responses to methamphetamine by active immunization with peptide-based, molecular adjuvant-containing vaccines

Michael J. Duryee, Rick A Bevins, Carmela M. Reichel, Jennifer E. Murray, Yuxiang Dong, Geoffrey Milton Thiele, Sam D. Sanderson

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Vaccines to methamphetamine (meth) were designed by covalently attaching a meth hapten (METH) to peptide constructs that contained a conformationally biased, response-selective molecular adjuvant, YSFKPMPLaR (EP54). Rats immunized with EP54-containing meth vaccines generated serum antibody titers to authentic meth, an immune outcome that altered meth self-administration. Immunization increased meth self-administration suggesting pharmacokinetic antagonism. The ability of immune sera to bind a METH-modified target protein dramatically decreased during and shortly after the meth self-administration assay, suggesting effective sequestration of free meth. However, the binding ability of immune sera to the METH-modified target protein was recovered 34 days after meth-free clearance time.

Original languageEnglish (US)
Pages (from-to)2981-2988
Number of pages8
JournalVaccine
Volume27
Issue number22
DOIs
StatePublished - May 14 2009

Fingerprint

Methamphetamine
adjuvants
antiserum
immunization
Vaccination
Vaccines
immune response
peptides
vaccines
haptens
Peptides
binding capacity
blood serum
pharmacokinetics
Self Administration
proteins
antibodies
rats
assays
Immune Sera

Keywords

  • Anti-methamphetamine antibodies
  • Conformationally biased
  • Methamphetamine vaccine
  • Molecular adjuvant
  • Response-selective C5a agonists
  • Self-administration

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Immune responses to methamphetamine by active immunization with peptide-based, molecular adjuvant-containing vaccines. / Duryee, Michael J.; Bevins, Rick A; Reichel, Carmela M.; Murray, Jennifer E.; Dong, Yuxiang; Thiele, Geoffrey Milton; Sanderson, Sam D.

In: Vaccine, Vol. 27, No. 22, 14.05.2009, p. 2981-2988.

Research output: Contribution to journalArticle

Duryee, Michael J. ; Bevins, Rick A ; Reichel, Carmela M. ; Murray, Jennifer E. ; Dong, Yuxiang ; Thiele, Geoffrey Milton ; Sanderson, Sam D. / Immune responses to methamphetamine by active immunization with peptide-based, molecular adjuvant-containing vaccines. In: Vaccine. 2009 ; Vol. 27, No. 22. pp. 2981-2988.
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