Immune responses following G-CSF mobilized allogeneic blood stem cell transplantation

Shantaram S Joshi, Steven Z. Pavletic, Stefano R. Tarantolo, James C. Lynch, Anne Kessinger, Michael R. Bishop

Research output: Contribution to journalArticle

Abstract

Rationale: Earlier research from this laboratory demonstrated a significant decrease in immune functions of mononuclear cells (MNC) from 104 G-CSF mobilized normal human donor blood stem cell harvests compared to steady state non-mobilized stem cell harvests. The clinical significance of these findings is not currently well understood. The studies on pattern of immune reconstitution in transplanted patients might be useful information to understand the biology/behavior of the transplanted cells in vivo. Objective: To evaluate the immune functions including natural killer (NK), lymphokine activated killer (LAK) cell mediated cytotoxicity, T and B lymphocyte response to specific mitogens and immune phenotypes of blood MNC from 104 patients transplanted with GCSF mobilized blood cells. Methods: In vitro immune responses were studied before starting the preparative regimen 28, 100, 200 and 365 days after transplantation. The results were compared to MNC from non-mobilized leukopheresis products and pretransplant baseline levels. Results: Normal Baseline Day 28 Day 100 Day 200 Day 365 NK Cytotoxicity 55±3.2 24+5.5@ 40.4±4 42.2±4.3 44.5±6.2 34±4@ LAK Cytotoxicity 36.7±4 16.1±6@ 32.513.5 27.2±5.4 26.916.4 20.913.6@ Con-A Response # 30,744 15,550@ 7,835 8,749 5,648 19,996@ PHA Response # 79,382 22.453@ 24,332@ 20,945(8 19,526@ 29,285@ LPS Response # 15.541 7,688@ 4,886 5,768 1,548 13,853@ PWM Response # 40,364 12,551@ 6,722 8,967 3,995 13,417Cytotoxicity at 50:1 Effector: Target Ratio; #: Mean counts per minute 3H-thymidine uptake @ : Significantly different from normal value; Significantly different from baseline value Summary/Conclusion:The results show that the antitumor cytotoxic functions are seen at a moderate levels during the post transplant immunohematological reconstitution. However, both T and B cell functions as measured by mitogen response do not come to baseline level till day 365 post transplant and did not come back to normal level during the study period. The decreased post immune functions might play a role in less than expected incidences of acute graft versus host disease observed in these patients following G-CSF mobilized stem cell transplantation.

Original languageEnglish (US)
Pages (from-to)355b
JournalBlood
Volume96
Issue number11 PART II
Publication statusPublished - Dec 1 2000

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Joshi, S. S., Pavletic, S. Z., Tarantolo, S. R., Lynch, J. C., Kessinger, A., & Bishop, M. R. (2000). Immune responses following G-CSF mobilized allogeneic blood stem cell transplantation. Blood, 96(11 PART II), 355b.